Understanding Bartonella-Associated Infective Endocarditis : Examining Heart Valve and Vegetation Appearance and the Role of Neutrophilic Leukocytes

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dc.contributor.authorMeidrops, Kristiāns
dc.contributor.authorGroma, Valērija
dc.contributor.authorGoldins, Niks Ricards
dc.contributor.authorApine, Lauma
dc.contributor.authorSkuja, Sandra
dc.contributor.authorSvirskis, Šimons
dc.contributor.authorGudrā, Dita
dc.contributor.authorFridmanis, Davids
dc.contributor.authorStradiņš, Pēteris
dc.contributor.institutionRīga Stradiņš University
dc.contributor.institutionJoint Laboratory of Electron Microscopy
dc.contributor.institutionInstitute of Microbiology and Virology
dc.contributor.institutionScientific Laboratory of Molecular Genetics
dc.date.accessioned2024-02-07T11:00:01Z
dc.date.available2024-02-07T11:00:01Z
dc.date.issued2024-01
dc.descriptionPublisher Copyright: © 2023 by the authors.
dc.description.abstractBackground. The endocardium and cardiac valves undergo severe impact during infective endocarditis (IE), and the formation of vegetation places IE patients at a heightened risk of embolic complications and mortality. The relevant literature indicates that 50% of IE cases exhibit structurally normal cardiac valves, with no preceding history of heart valve disease. Gram-positive cocci emerge as the predominant causative microorganisms in IE, while Gram-negative Bartonella spp., persisting in the endothelium, follow pathogenic pathways distinct from those of typical IE-causing agents. Employing clinical as well as advanced microbiological and molecular assays facilitated the identification of causative pathogens, and various morphological methods were applied to evaluate heart valve damage, shedding light on the role of neutrophilic leukocytes in host defense. In this research, the immunohistochemical analysis of neutrophilic leukocyte activation markers such as myeloperoxidase, neutrophil elastase, calprotectin, and histone H3, was performed. A distinct difference in the expression patterns of these markers was observed when comparing Bartonella spp.-caused and non-Bartonella spp.-caused IE. The markers exhibited significantly higher expression in non-Bartonella spp.-caused IE compared to Bartonella spp.-caused IE, and they were more prevalent in vegetation than in the valvular leaflets. Notably, the expression of these markers in all IE cases significantly differed from that in control samples. Furthermore, we advocated the use of 16S rRNA Next-Generation Sequencing on excised heart valves as an effective diagnostic tool for IE, particularly in cases where blood cultures yielded negative results. The compelling results achieved in this study regarding the enigmatic nature of Bartonella spp. IE’s pathophysiology contribute significantly to our understanding of the peculiarities of inflammation and immune responses.en
dc.description.statusPeer reviewed
dc.format.extent28
dc.format.extent10574717
dc.identifier.citationMeidrops, K, Groma, V, Goldins, N R, Apine, L, Skuja, S, Svirskis, Š, Gudrā, D, Fridmanis, D & Stradiņš, P 2024, 'Understanding Bartonella-Associated Infective Endocarditis : Examining Heart Valve and Vegetation Appearance and the Role of Neutrophilic Leukocytes', Cells, vol. 13, no. 1, 43. https://doi.org/10.3390/cells13010043, https://doi.org/10.3390/cells13010043
dc.identifier.doi10.3390/cells13010043
dc.identifier.issn2073-4409
dc.identifier.otherMendeley: 9105d890-8554-38a2-9fc4-1d34063d0db9
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/15153
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85181960575&partnerID=8YFLogxK
dc.identifier.urlhttps://www.mendeley.com/catalogue/9105d890-8554-38a2-9fc4-1d34063d0db9/
dc.language.isoeng
dc.relation.ispartofCells
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBartonellaspp
dc.subjectcardiac valves
dc.subjectinfective endocarditis
dc.subjectneutrophil extracellular traps
dc.subjectneutrophils
dc.subjectvegetation
dc.subjectBartonella
dc.subjectHeart Valves
dc.subjectHumans
dc.subjectLeukocytes
dc.subjectEndocarditis, Bacterial
dc.subjectEndocarditis
dc.subjectRNA, Ribosomal, 16S
dc.subject3.2 Clinical medicine
dc.subject3.1 Basic medicine
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectGeneral Biochemistry,Genetics and Molecular Biology
dc.titleUnderstanding Bartonella-Associated Infective Endocarditis : Examining Heart Valve and Vegetation Appearance and the Role of Neutrophilic Leukocytesen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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