Composition of mastitis causing microorganisms and cytokines in healthy cow’s milk : a pilot study

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dc.contributor.authorJunga, Anna
dc.contributor.authorPilmane, Māra
dc.contributor.authorŠerstņova, Ksenija
dc.contributor.authorLohova, Elizabeta
dc.contributor.authorMelderis, Ivars
dc.contributor.authorGontars, Lukašs
dc.contributor.authorKochanski, Maksymilian
dc.contributor.authorDrutowska, Andzelika
dc.contributor.authorGergely, Maroti
dc.contributor.authorPrieto-Simona, Beatrise
dc.contributor.institutionInstitute of Anatomy and Anthropology
dc.date.accessioned2023-10-06T12:50:01Z
dc.date.available2023-10-06T12:50:01Z
dc.date.issued2023
dc.descriptionPublisher Copyright: © 2023 Anna Junga et al., published by Sciendo.
dc.description.abstractThe aim of this study was to examine clinically healthy cow’s udder milk microbiota and presence of cytokines in different seasons. Milk samples taken from the cows were checked for the presence of Gram-positive and Gram-negative bacteria, and the somatic cell count was detected. Immunohistochemistry methods were performed to detect interleukin (IL) -2, IL-4, IL-8, IL-10, IL-12, IL-17a, â-defensin-3, transforming growth factor (TGF)-â1, interferon-ã and nuclear factor (NF)-êB presence in the milk. S. agalactiae, S. uberis, S. aureus, E. coli, and Klebsiella, Enterobacter, Citrobacter spp. were found in healthy cow’s milk. In the first round, the highest prevalence was observed for S. aureus. In the second round, the highest mean levels were observed for S. uberis, then followed S. aureus. IL-4, IL-17a and TGF-â1 demonstrated the highest expression in the milk samples. NF-êB had the lowest expression among all factors. The presence of a rich bacterial microbiome (mostly S.aureus, S.uberis) in the milk of healthy animals, as well as changing bacterial species between in spring and autumn seasons occur as a result of both the immune state of the animal and many external factors, which consequently affects the amount of expressed cytokines.en
dc.description.statusPeer reviewed
dc.format.extent9
dc.format.extent1664112
dc.identifier.citationJunga, A, Pilmane, M, Šerstņova, K, Lohova, E, Melderis, I, Gontars, L, Kochanski, M, Drutowska, A, Gergely, M & Prieto-Simona, B 2023, 'Composition of mastitis causing microorganisms and cytokines in healthy cow’s milk : a pilot study', Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences., vol. 77, no. 3-4, pp. 169-177. https://doi.org/10.2478/prolas-2023-0024
dc.identifier.doi10.2478/prolas-2023-0024
dc.identifier.issn2255-890X
dc.identifier.otherMendeley: e17bea88-4d9a-3c6f-89c9-203eea43badc
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/14911
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85174387491&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofProceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectmicrobiota
dc.subjectinterleukins
dc.subjectimmunocytochemistry
dc.subjectmicroscopy
dc.subjectgrowth factors
dc.subject3.1 Basic medicine
dc.subject3.3 Health sciences
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.titleComposition of mastitis causing microorganisms and cytokines in healthy cow’s milk : a pilot studyen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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