Upregulation of the chemokine receptor CCR2B in Epstein-Barr Virus-positive Burkitt lymphoma cell lines with the latency III program
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2018-05-03
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Abstract
CCR2 is the cognate receptor to the chemokine CCL2. CCR2–CCL2 signaling mediates cancer progression and metastasis dissemination. However, the role of CCR2–CCL2 signaling in pathogenesis of B-cell malignancies is not clear. Previously, we showed that CCR2B was upregulated in ex vivo peripheral blood B cells upon Epstein-Barr virus (EBV) infection and in established lymphoblastoid cell lines with the EBV latency III program. EBV latency III is associated with B-cell lymphomas in immunosuppressed patients. The majority of EBV-positive Burkitt lymphoma (BL) tumors are characterized by latency I, but the BL cell lines drift towards latency III during in vitro culture. In this study, the CCR2A and CCR2B expression was assessed in the isogenic EBV-positive BL cell lines with latency I and III using RT-PCR, immunoblotting, and immunostaining analyses. We found that CCR2B is upregulated in the EBV-positive BL cells with latency III. Consequently, we detected the migration of latency III cells toward CCL2. Notably, the G190A mutation, corresponding to SNP CCR2-V64I, was found in one latency III cell line with a reduced migratory response to CCL2. The upregulation of CCR2B may contribute to the enhanced migration of malignant B cells into CCL2-rich compartments.
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Funding Information: Acknowledgments: The study was supported by the Latvian Council of Science project No. 651/2014 and visit grants from the FP7-REGPOT-2012-2013-1 HEALTH Call Program Baltinfect project (EU Grant agreement No. 316275). Publisher Copyright: © 2018 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.
Keywords
B-cell lymphoma, Burkitt lymphoma cell lines, CCR2, EBV, Latency III, 3.2 Clinical medicine, 1.1. Scientific article indexed in Web of Science and/or Scopus database, Infectious Diseases, Virology, SDG 3 - Good Health and Well-being
Citation
Kozireva, S, Rudevica, Z, Baryshev, M, Leonciks, A, Kashuba, E & Kholodnyuk, I 2018, 'Upregulation of the chemokine receptor CCR2B in Epstein-Barr Virus-positive Burkitt lymphoma cell lines with the latency III program', Viruses, vol. 10, no. 5, 239. https://doi.org/10.3390/v10050239