Co‐occurrence of free‐living amoeba and legionella in drinking water supply systems

Simple item page
dc.contributor.authorValciņa, Olga
dc.contributor.authorPūle, Daina
dc.contributor.authorMališevs, Artjoms
dc.contributor.authorTrofimova, Jūlija
dc.contributor.authorMakarova, Svetlana
dc.contributor.authorKonvisers, Genadijs
dc.contributor.authorBērziņš, Aivars
dc.contributor.authorKrūmiņa, Angelika
dc.contributor.institutionDepartment of Infectology
dc.date.accessioned2021-04-28T08:20:01Z
dc.date.available2021-04-28T08:20:01Z
dc.date.issued2019-08
dc.descriptionFunding Information: Funding: This study was funded by National Research Programme No. 7, Agricultural Resources for Sustainable Production of Qualitative and Healthy Foods in Latvia project No. 5 “Resistance of microorganisms and other biological and chemical risks: research procedure development and application in the food chain.” Publisher Copyright: © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
dc.description.abstractBackground and Objectives: Legionella is one of the most important water-related pathogens. Inside the water supply systems and the biofilms, Legionella interact with other bacteria and free-living amoeba (FLA). Several amoebas may serve as hosts for bacteria in aquatic systems. This study aimed to investigate the co-occurrence of Legionella spp. and FLA in drinking water supply systems. Materials and Methods: A total of 268 water samples were collected from apartment buildings, hotels, and public buildings. Detection of Legionella spp. was performed in accordance with ISO 11731:2017 standard. Three different polymerase chain reaction (PCR) protocols were used to identify FLA. Results: Occurrence of Legionella varied from an average of 12.5% in cold water samples with the most frequent occurrence observed in hot water, in areas receiving untreated groundwater, where 54.0% of the samples were Legionella positive. The occurrence of FLA was significantly higher. On average, 77.2% of samples contained at least one genus of FLA and, depending on the type of sample, the occurrence of FLA could reach 95%. In the samples collected during the study, Legionella was always isolated along with FLA, no samples containing Legionella in the absence of FLA were observed. Conclusions: The data obtained in our study can help to focus on the extensive distribution, close interaction, and long-term persistence of Legionella and FLA. Lack of Legionella risk management plans and control procedures may promote further spread of Legionella in water supply systems. In addition, the high incidence of Legionella-related FLA suggests that traditional monitoring methods may not be sufficient for Legionella control.en
dc.description.statusPeer reviewed
dc.format.extent1054483
dc.identifier.citationValciņa, O, Pūle, D, Mališevs, A, Trofimova, J, Makarova, S, Konvisers, G, Bērziņš, A & Krūmiņa, A 2019, 'Co‐occurrence of free‐living amoeba and legionella in drinking water supply systems', Medicina (Lithuania), vol. 55, no. 8, 492. https://doi.org/10.3390/medicina55080492
dc.identifier.doi10.3390/medicina55080492
dc.identifier.issn1010-660X
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/3951
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85071489289&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofMedicina (Lithuania)
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAmoeba
dc.subjectCo-occurrence
dc.subjectLegionella
dc.subjectProtozoa
dc.subjectWater
dc.subject3.1 Basic medicine
dc.subject3.2 Clinical medicine
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectGeneral Medicine
dc.titleCo‐occurrence of free‐living amoeba and legionella in drinking water supply systemsen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Co_Occurrence_of_Free_Living_Amoeba_and_Legionella.pdf
Size:
1.01 MB
Format:
Adobe Portable Document Format
Download

Collections

Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure