The Cultivation of Chelidonium majus L. Increased the Total Alkaloid Content and Cytotoxic Activity Compared with Those of Wild-Grown Plants

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dc.contributor.authorKrizhanovska, Valerija
dc.contributor.authorSile, Inga
dc.contributor.authorKronberga, Arta
dc.contributor.authorNakurte, Ilva
dc.contributor.authorMezaka, Ieva
dc.contributor.authorDambrova, Maija
dc.contributor.authorPugovics, Osvalds
dc.contributor.authorGrinberga, Solveiga
dc.contributor.institutionDepartment of Applied Pharmacy
dc.contributor.institutionDepartment of Pharmaceutical Chemistry
dc.date.accessioned2021-09-28T08:05:01Z
dc.date.available2021-09-28T08:05:01Z
dc.date.issued2021-09
dc.descriptionFunding Information: Funding: This research was funded by European Regional Development Fund project “Innovative solutions for growing technologies and applications of spring medicinal and aromatic plants” (Grant No. 1.1.1.1/18/A/ 043). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
dc.description.abstractThe effect of cultivation practises on both the phytochemical profile and biological activity of aqueous ethanol extracts of Chelidonium majus L. was studied. Extracts were prepared from aerial parts of the same plant population collected in the wild and grown under organic farming conditions. Both qualitative and quantitative analyses of alkaloids and flavonoid derivatives were performed by LC/MS methods, and the cytotoxicity of lyophilised extracts was studied in B16-F10, HepG2, and CaCo-2 cells. Coptisine was the dominant alkaloid of extracts prepared from wild-grown plants, whereas after cultivation, chelidonine was the most abundant alkaloid. The total alkaloid content was significantly increased by cultivation. Ten flavonol glycoconjugates were identified in C. majus extracts, and quantitative analysis did not reveal significant differences between extracts prepared from wild-grown and cultivated specimens. Treatment with C. majus extracts resulted in a dose-dependent increase in cytotoxicity in all three cell lines. The extracts prepared from cultivated specimens showed higher cytotoxicity than the extracts prepared from wild-grown plants. The strongest cytotoxic effect of cultivated C. majus was observed in B16-F10 cells (IC50 = 174.98 ± 1.12 µg/mL). Cultivation-induced differences in the phytochemical composition of C. majus extracts resulted in significant increases in the cytotoxic activities of the preparations.en
dc.description.statusPeer reviewed
dc.format.extent653757
dc.identifier.citationKrizhanovska, V, Sile, I, Kronberga, A, Nakurte, I, Mezaka, I, Dambrova, M, Pugovics, O & Grinberga, S 2021, 'The Cultivation of Chelidonium majus L. Increased the Total Alkaloid Content and Cytotoxic Activity Compared with Those of Wild-Grown Plants', Plants, vol. 10, no. 9, 1971. https://doi.org/10.3390/plants10091971
dc.identifier.doi10.3390/plants10091971
dc.identifier.issn2223-7747
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/6451
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85115203247&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofPlants
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAlkaloids
dc.subjectChelidonium majus
dc.subjectCytotoxic activity
dc.subjectFlavonoids
dc.subjectLC/MS analysis
dc.subject3.1 Basic medicine
dc.subject1.6 Biological sciences
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectEcology, Evolution, Behavior and Systematics
dc.subjectEcology
dc.subjectPlant Science
dc.titleThe Cultivation of Chelidonium majus L. Increased the Total Alkaloid Content and Cytotoxic Activity Compared with Those of Wild-Grown Plantsen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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