Oregonin from Alnus incana bark affects DNA methyltransferases expression and mitochondrial DNA copies in mouse embryonic fibroblasts

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dc.contributor.authorKrasilnikova, Jelena
dc.contributor.authorLauberte, Liga
dc.contributor.authorStoyanova, Elena
dc.contributor.authorAbadjieva, Desislava
dc.contributor.authorChervenkov, Mihail
dc.contributor.authorMori, Mattia
dc.contributor.authorDe Paolis, Elisa
dc.contributor.authorMladenova, Vanya
dc.contributor.authorTelysheva, Galina
dc.contributor.authorBotta, Bruno
dc.contributor.authorKistanova, Elena
dc.contributor.institutionDepartment of Human Physiology and Biochemistry
dc.date.accessioned2021-07-05T12:55:01Z
dc.date.available2021-07-05T12:55:01Z
dc.date.issued2018-01-01
dc.descriptionFunding Information: This work was supported by National Scientific Fund of Bulgaria [Grant DKOST01/10]. The research was implemented under frame of collaboration in COST Action FA1403 and bilateral project between Bulgarian Academy of Sciences and Latvian Academy of Sciences. We gratitude doctor M. Kicheva from “Progene-Ltd” company for technical support of the gene expression and mtDNA copy research. Funding Information: This work was supported by National Scientific Fund of Bulgaria [Grant DKOST01/10]. Publisher Copyright: © 2018, © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
dc.description.abstractOregonin is an open-chain diarylheptanoid isolated from Alnus incana bark that possesses remarkable antioxidant and anti-inflammatory properties, inhibits adipogenesis, and can be used in the prevention of obesity and related metabolic disorders. Here, we aimed to investigate the effects of oregonin on the epigenetic regulation in cells as well as its ability to modulate DNA methylating enzymes expression and mitochondrial DNA (mtDNA) copies. Our results show that oregonin altered the expression of DNA methyltransferases and mtDNA copy numbers in dependency on concentration and specificity of cells genotype. A close correlation between mtDNA copy numbers and mRNA expression of the mtDnmt1 and Dnmt3b was established. Moreover, molecular modeling suggested that oregonin fits the catalytic site of DNMT1 and partially overlaps with binding of the cofactor. These findings further extend the knowledge on oregonin, and elucidate for the first time its potential to affect the key players of the DNA methylation process, namely DNMTs transcripts and mtDNA.en
dc.description.statusPeer reviewed
dc.format.extent9
dc.format.extent1837449
dc.identifier.citationKrasilnikova, J, Lauberte, L, Stoyanova, E, Abadjieva, D, Chervenkov, M, Mori, M, De Paolis, E, Mladenova, V, Telysheva, G, Botta, B & Kistanova, E 2018, 'Oregonin from Alnus incana bark affects DNA methyltransferases expression and mitochondrial DNA copies in mouse embryonic fibroblasts', Journal of Enzyme Inhibition and Medicinal Chemistry, vol. 33, no. 1, pp. 1055-1063. https://doi.org/10.1080/14756366.2018.1476504
dc.identifier.doi10.1080/14756366.2018.1476504
dc.identifier.issn1475-6366
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/5829
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85048256882&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofJournal of Enzyme Inhibition and Medicinal Chemistry
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAlnus incana bark
dc.subjectDNA methyltransferases mRNAs
dc.subjectmtDNA copy
dc.subjectOregonin
dc.subject1.6 Biological sciences
dc.subject3.1 Basic medicine
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectPharmacology
dc.subjectDrug Discovery
dc.subjectSDG 3 - Good Health and Well-being
dc.titleOregonin from Alnus incana bark affects DNA methyltransferases expression and mitochondrial DNA copies in mouse embryonic fibroblastsen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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