Inflammatory cytokine-producing cells and inflammation markers in the synovium of osteoarthritis patients evidenced in human herpesvirus 7 infection

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dc.contributor.authorGroma, Valerija
dc.contributor.authorTarasovs, Mihails
dc.contributor.authorSkuja, Sandra
dc.contributor.authorSemenistaja, Sofija
dc.contributor.authorNora-Krukle, Zaiga
dc.contributor.authorSvirskis, Simons
dc.contributor.authorMurovska, Modra
dc.contributor.institutionJoint Laboratory of Electron Microscopy
dc.contributor.institutionInstitute of Anatomy and Anthropology
dc.contributor.institutionDepartment of Internal Diseases
dc.contributor.institutionInstitute of Microbiology and Virology
dc.date.accessioned2021-09-06T12:40:01Z
dc.date.available2021-09-06T12:40:01Z
dc.date.issued2020-09-01
dc.descriptionFunding Information: Acknowledgments: This study was supported by the Latvian Council of Science Grant Nr. Izp-2018/1-0149 and the National Research Programme Biomedicine for the Public Health (BIOMEDICINE), project 7.2. The authors would like to thank Anda Kadisa, Rheumatologist at Riga East University Hospital Clinic “Gailezers”, Latvia, for advising on the diagnosis and recruitment of osteoarthritis patients. Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.
dc.description.abstractA direct association between joint inflammation and the progression of osteoarthritis (OA) has been proposed, and synovitis is considered a powerful driver of the disease. Among infections implicated in the development of joint disease, human herpesvirus 7 (HHV-7) infection remains poorly characterized. Therefore, we assessed synovitis in OA patients; determined the occurrence and distribution of the HHV-7 antigen within the synovial membrane of OA-affected subjects; and correlated plasma levels of the pro-inflammatory cytokines tumor necrosis factor (TNF), interleukin-6 (IL-6), and TNF expressed locally within lesioned synovial tissues with HHV-7 observations, suggesting differences in persistent latent and active infection. Synovial HHV-7, CD4, CD68, and TNF antigens were detected immunohistochemically. The plasma levels of TNF and IL6 were measured by an enzyme-linked immunosorbent assay. Our findings confirm the presence of persistent HHV-7 infection in 81.5% and reactivation in 20.5% of patients. In 35.2% of patients, virusspecific DNA was extracted from synovial membrane tissue samples. We evidenced the absence of histopathologically detectable synovitis and low-grade changes in the majority of OA patients enrolled in the study, in both HHV-7 PCR+ and HHV-7 PCR‒ groups. The number of synovial CD4positive cells in the HHV-7 polymerase chain reaction (PCR)+ group was significantly higher than that in the HHV-7 PCR‒ group. CD4-and CD68-positive cells were differently distributed in both HHV-7 PCR+ and HHV-7 PCR‒ groups, as well as in latent and active HHV-7 infection. The number of TNF+ and HHV-7+ lymphocytes, as well as HHV-7+ vascular endothelial cells, was strongly correlated. Vascular endothelial cells, especially in the case of infection reactivation, appeared vulnerable. The balance between virus latency and reactivation is a long-term relationship between the host and infectious agent, and the immune system appears to be involved in displaying overreaction when a shift in the established equilibrium develops.en
dc.description.statusPeer reviewed
dc.format.extent18
dc.format.extent3693222
dc.identifier.citationGroma, V, Tarasovs, M, Skuja, S, Semenistaja, S, Nora-Krukle, Z, Svirskis, S & Murovska, M 2020, 'Inflammatory cytokine-producing cells and inflammation markers in the synovium of osteoarthritis patients evidenced in human herpesvirus 7 infection', International Journal of Molecular Sciences, vol. 21, no. 17, 6004, pp. 1-18. https://doi.org/10.3390/ijms21176004
dc.identifier.doi10.3390/ijms21176004
dc.identifier.issn1661-6596
dc.identifier.otherunpaywall: 10.3390/ijms21176004
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/6196
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85089652321&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCytokines
dc.subjectELISA
dc.subjectHHV-7
dc.subjectImmunohistochemistry
dc.subjectOsteoarthritis
dc.subjectPCR
dc.subjectSynovium
dc.subject2.4 Chemical engineering
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectCatalysis
dc.subjectMolecular Biology
dc.subjectSpectroscopy
dc.subjectComputer Science Applications
dc.subjectPhysical and Theoretical Chemistry
dc.subjectOrganic Chemistry
dc.subjectInorganic Chemistry
dc.subjectSDG 3 - Good Health and Well-being
dc.titleInflammatory cytokine-producing cells and inflammation markers in the synovium of osteoarthritis patients evidenced in human herpesvirus 7 infectionen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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