Frequency and significance of parvovirus B19 infection in patients with rheumatoid arthritis

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dc.contributor.authorNaciute, Milda
dc.contributor.authorMieliauskaite, Diana
dc.contributor.authorRugiene, Rita
dc.contributor.authorNikitenkiene, Rita
dc.contributor.authorJancoriene, Ligita
dc.contributor.authorMauricas, Mykolas
dc.contributor.authorNora-Krukle, Zaiga
dc.contributor.authorMurovska, Modra
dc.contributor.authorGirkontaite, Irute
dc.contributor.institutionInstitute of Microbiology and Virology
dc.date.accessioned2021-04-20T13:05:02Z
dc.date.available2021-04-20T13:05:02Z
dc.date.issued2016-12
dc.descriptionFunding Information: This work was supported in part by the Research Council of Lithuania (TAP LLT 02/201), the Taiwan-Latvia-Lithuania Cooperation project 2012-2014 and 7FP project ?Unlocking infectious diseases research potential at Riga Stradn? University?, Baltinfect, agreement number 316275. We thank Professor Derek Pheby, Visiting Professor of Epidemiology at Buckinghamshire New University, UK, for advising on the use of English in this paper Publisher Copyright: © 2016 The Authors. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.
dc.description.abstractThe present study aims to clarify the possible involvement of parvovirus B19 (B19V) infection in rheumatoid arthritis (RA) pathogenesis by investigating the presence of B19V infection markers (genomic sequences and virus-specific antibodies) in association with the level of cytokines and RA clinical activity and aggressiveness. A total of 118 RA patients and 49 ageand sex-matched healthy volunteers were enrolled in the study. Nested PCR was used to detect B19V sequences in whole blood and cell-free plasma DNA, ELISA to detect virusspecific antibodies and cytokine levels in plasma and recomLine dot blot assay for antibodies to separate B19V antigens. The detection frequency of B19V DNA was higher in patients with RA (25.4%) in comparison with healthy persons (18.4%). B19V DNA in cell-free plasma (B19+p) was detected significantly often in RA patients in comparison with healthy controls (13.6 vs 2%; P=0.0002). RA B19+p patients had higher disease activity and aggressiveness, decreased haemoglobin and increased erythrocyte sedimentation rates. IL-6 plasma levels were significantly higher in RA patients than in controls. Within the RA patients’ group the IL-6 level was significantly increased in B19+p patients with disease activity scores of DAS28>5.2, high C-reactive protein and low haemoglobin. Contrary to the healthy controls, the majority of RA B19+p patients did not have antibodies to VP-1S (VP1u) and VP-N (N-terminal half of structural proteins VP1 and VP2), which correspond to the epitopes of neutralizing antibodies. These results indicate that B19V infection at least in some patients is involved in RA pathogenesis.en
dc.description.statusPeer reviewed
dc.format.extent11
dc.format.extent262199
dc.identifier.citationNaciute, M, Mieliauskaite, D, Rugiene, R, Nikitenkiene, R, Jancoriene, L, Mauricas, M, Nora-Krukle, Z, Murovska, M & Girkontaite, I 2016, 'Frequency and significance of parvovirus B19 infection in patients with rheumatoid arthritis', Journal of General Virology, vol. 97, no. 12, 000621, pp. 3302-3312. https://doi.org/10.1099/jgv.0.000621
dc.identifier.doi10.1099/jgv.0.000621
dc.identifier.issn0022-1317
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/3868
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85007223349&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofJournal of General Virology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectAntibodies
dc.subjectIL-6
dc.subjectParvovirus B19
dc.subjectRheumatoid arthritis
dc.subject1.6 Biological sciences
dc.subject3.3 Health sciences
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectVirology
dc.titleFrequency and significance of parvovirus B19 infection in patients with rheumatoid arthritisen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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