Identification of High-Risk Human Papillomavirus DNA, p16, and E6/E7 Oncoproteins in Laryngeal and Hypopharyngeal Squamous Cell Carcinomas

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dc.contributor.authorLifsics, Andrejs
dc.contributor.authorGroma, Valerija
dc.contributor.authorCistjakovs, Maksims
dc.contributor.authorSkuja, Sandra
dc.contributor.authorDeksnis, Renars
dc.contributor.authorMurovska, Modra
dc.contributor.institutionDepartment of Otorhinolaryngology
dc.contributor.institutionInstitute of Anatomy and Anthropology
dc.contributor.institutionInstitute of Microbiology and Virology
dc.date.accessioned2021-06-13T08:50:01Z
dc.date.available2021-06-13T08:50:01Z
dc.date.issued2021-06
dc.descriptionFunding Information: The authors would like to thank the Riga Stradi?? University Department of Doctoral studies for reagents and publishing support. We would also like to thank Elza Rate, MD at the Department of Head and Neck Surgery, Oncology Centre of Latvia, for additional support with the collection of some tumor specimens. We are grateful to Simons Svirskis for his valuable help with statistical analysis. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
dc.description.abstractHuman papillomavirus (HPV) was proven to play a significant role in cancer development in the oropharynx. However, its role in the development of laryngeal (LSCC) and hypopharyngeal squamous cell carcinoma (HPSCC) remains to be clarified. High-risk HPV (HR-HPV) viral proteins E6 and E7 are considered to be pertinent to HPV-related carcinogenesis. Hence, our aim was to estimate LSCC and HPSCC for HR-HPV DNA, p16, and E6/E7 oncoprotein status by using molecular virology and immunohistochemistry methods. The prevalence of HPV16 infection was 22/41 (53.7%) and 20/31 (64.5%) for LSCC and HPSCC, accordingly. The majority of HPV16+ tumor samples were stage III or IV. In most samples, the presence of either HPV16 E6 or HPV16 E7 viral protein in dysplastic or tumor cells was confirmed using immunohistochemistry. Our results suggest a high prevalence of HPV16 as a primary HR-HPV type in LSCC and HPSCC. The lack of HPV E6/E7 oncoproteins in some tumor samples may suggest either the absence of viral integration or the presence of other mechanisms of tumorigenesis. The utilization of p16 IHC as a surrogate marker of HR-HPV infection is impractical in LSCC and HPSCC.en
dc.description.statusPeer reviewed
dc.format.extent17
dc.format.extent7297956
dc.identifier.citationLifsics, A, Groma, V, Cistjakovs, M, Skuja, S, Deksnis, R & Murovska, M 2021, 'Identification of High-Risk Human Papillomavirus DNA, p16, and E6/E7 Oncoproteins in Laryngeal and Hypopharyngeal Squamous Cell Carcinomas', Viruses, vol. 13, no. 6, 1008. https://doi.org/10.3390/v13061008
dc.identifier.doi10.3390/v13061008
dc.identifier.issn1999-4915
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/4935
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85107442330&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofViruses
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectHPV
dc.subjectlarynx
dc.subjecthypopharynx
dc.subjectPCR
dc.subjectsquamous cell carcinoma
dc.subjectimmunohistochemistry
dc.subjectP16 Regulatory protein
dc.subjectE6/E7 viral oncoproteins
dc.subject3.1 Basic medicine
dc.subject3.2 Clinical medicine
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectSDG 3 - Good Health and Well-being
dc.titleIdentification of High-Risk Human Papillomavirus DNA, p16, and E6/E7 Oncoproteins in Laryngeal and Hypopharyngeal Squamous Cell Carcinomasen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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