Comparative Immunogenicity in Rabbits of the Polypeptides Encoded by the 5′ Terminus of Hepatitis C Virus RNA

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dc.contributor.authorSominskaya, Irina
dc.contributor.authorJansons, Juris
dc.contributor.authorDovbenko, Anastasija
dc.contributor.authorPetrakova, Natalia
dc.contributor.authorLieknina, Ilva
dc.contributor.authorMihailova, Marija
dc.contributor.authorLatyshev, Oleg
dc.contributor.authorEliseeva, Olesja
dc.contributor.authorStahovska, Irina
dc.contributor.authorAkopjana, Inara
dc.contributor.authorPetrovskis, Ivars
dc.contributor.authorIsaguliants, Maria
dc.contributor.institutionRīga Stradiņš University
dc.date.accessioned2021-07-02T09:45:01Z
dc.date.available2021-07-02T09:45:01Z
dc.date.issued2015
dc.descriptionPublisher Copyright: © 2015 Irina Sominskaya et al.
dc.description.abstractRecent studies on the primate protection from HCV infection stressed the importance of immune response against structural viral proteins. Strong immune response against nucleocapsid (core) protein was difficult to achieve, requesting further experimentation in large animals. Here, we analyzed the immunogenicity of core aa 1-173, 1-152, and 147-191 and of its main alternative reading frame product F-protein in rabbits. Core aa 147-191 was synthesized; other polypeptides were obtained by expression in E. coli. Rabbits were immunized by polypeptide primes followed by multiple boosts and screened for specific anti-protein and anti-peptide antibodies. Antibody titers to core aa 147-191 reached 105; core aa 1-152, 5 × 105; core aa 1-173 and F-protein, 106. Strong immunogenicity of the last two proteins indicated that they may compete for the induction of immune response. The C-terminally truncated core was also weakly immunogenic on the T-cell level. To enhance core-specific cellular response, we immunized rabbits with the core aa 1-152 gene forbidding F-protein formation. Repeated DNA immunization induced a weak antibody and sustained proliferative response of broad specificity confirming a gain of cellular immunogenicity. Epitopes recognized in rabbits overlapped those in HCV infection. Our data promotes the use of rabbits for the immunogenicity tests of prototype HCV vaccines.en
dc.description.statusPeer reviewed
dc.format.extent2012704
dc.identifier.citationSominskaya, I, Jansons, J, Dovbenko, A, Petrakova, N, Lieknina, I, Mihailova, M, Latyshev, O, Eliseeva, O, Stahovska, I, Akopjana, I, Petrovskis, I & Isaguliants, M 2015, 'Comparative Immunogenicity in Rabbits of the Polypeptides Encoded by the 5′ Terminus of Hepatitis C Virus RNA', Journal of Immunology Research, vol. 2015, 762426. https://doi.org/10.1155/2015/762426
dc.identifier.doi10.1155/2015/762426
dc.identifier.issn2314-8861
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/5817
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=84947460597&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofJournal of Immunology Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subject1.6 Biological sciences
dc.subject3.1 Basic medicine
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectImmunology and Allergy
dc.subjectImmunology
dc.subjectSDG 3 - Good Health and Well-being
dc.titleComparative Immunogenicity in Rabbits of the Polypeptides Encoded by the 5′ Terminus of Hepatitis C Virus RNAen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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