A 5-transcript signature for discriminating viral and bacterial etiology in pediatric pneumonia

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dc.contributor.authorViz-Lasheras, Sandra
dc.contributor.authorGómez-Carballa, Alberto
dc.contributor.authorPardo-Seco, Jacobo
dc.contributor.authorZavadska, Dace
dc.contributor.authorDIAMONDS
dc.contributor.authorGENDRES
dc.contributor.authorPERFORM consortia
dc.contributor.institutionRīga Stradiņš University
dc.date.accessioned2025-02-05T10:15:01Z
dc.date.available2025-02-05T10:15:01Z
dc.date.issued2025-02-21
dc.descriptionPublisher Copyright: © 2025 The Author(s)
dc.description.abstractPneumonia stands as the primary cause of death among children under five, yet current diagnosis methods often result in inadequate or unnecessary treatments. Our research seeks to address this gap by identifying host transcriptomic biomarkers in the blood of children with definitive viral and bacterial pneumonia. We performed RNA sequencing on 192 prospectively collected whole blood samples, including 38 controls and 154 pneumonia cases, uncovering a 5-transcript signature (genes FAM20A, BAG3, TDRD9, MXRA7, and KLF14) that effectively distinguishes bacterial from viral pneumonia (area under the curve (AUC): 0.95 [0.88–1.00]). Initial validation using combined definitive and probable cases yielded an AUC of 0.87 [0.77–0.97], while full validation in a new prospective cohort of 32 patients achieved an AUC of 0.92 [0.83–1.00]. This robust signature holds significant potential to enhance diagnostics accuracy for pediatric pneumonia, reducing diagnostic delays and unnecessary treatments and potentially transforming clinical practice.en
dc.description.statusPeer reviewed
dc.format.extent5932114
dc.identifier.citationViz-Lasheras, S, Gómez-Carballa, A, Pardo-Seco, J, Zavadska, D, DIAMONDS, GENDRES & PERFORM consortia 2025, 'A 5-transcript signature for discriminating viral and bacterial etiology in pediatric pneumonia', iScience, vol. 28, no. 2, 111747. https://doi.org/10.1016/j.isci.2025.111747
dc.identifier.doi10.1016/j.isci.2025.111747
dc.identifier.issn2589-0042
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/17073
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85215439484&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofiScience
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBody substance sample
dc.subjectClinical microbiology
dc.subjectDiagnostics
dc.subjectPediatrics
dc.subjectTranscriptomics
dc.subject3.2 Clinical medicine
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectGeneral
dc.titleA 5-transcript signature for discriminating viral and bacterial etiology in pediatric pneumoniaen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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