Ocular Surface Microbiota in Naïve Keratoconus : A Multicenter Validation Study

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dc.contributor.authorRocha-de-Lossada, Carlos
dc.contributor.authorMazzotta, Cosimo
dc.contributor.authorGabrielli, Federico
dc.contributor.authorPapa, Filomena Tiziana
dc.contributor.authorGómez-Huertas, Carmen
dc.contributor.authorGarcía-López, Celia
dc.contributor.authorUrbinati, Facundo
dc.contributor.authorRachwani-Anil, Rahul
dc.contributor.authorGarcía-Lorente, María
dc.contributor.authorSánchez-González, José María
dc.contributor.authorRechichi, Miguel
dc.contributor.authorRubegni, Giovanni
dc.contributor.authorBorroni, Davide
dc.date.accessioned2025-01-02T11:10:01Z
dc.date.available2025-01-02T11:10:01Z
dc.date.issued2023-10
dc.descriptionPublisher Copyright: © 2023 by the authors.
dc.description.abstractIn the field of Ophthalmology, the mNGS 16S rRNA sequencing method of studying the microbiota and ocular microbiome is gaining more and more weight in the scientific community. This study aims to characterize the ocular microbiota of patients diagnosed with keratoconus who have not undergone any prior surgical treatment using the mNGS 16S rRNA sequencing method. Samples of naïve keratoconus patients were collected with an eNAT with 1 mL of Liquid Amies Medium (Copan Brescia, Italy), and DNA was extracted and analyzed with 16S NGS. The microbiota analysis showed a relative abundance of microorganisms at the phylum level in each sample collected from 38 patients with KC and 167 healthy controls. A comparison between healthy control and keratoconus samples identified two genera unique to keratoconus, Pelomonas and Ralstonia. Our findings suggest that alterations in the microbiota may play a role in the complex scenario of KC development.en
dc.description.statusPeer reviewed
dc.format.extent2239769
dc.identifier.citationRocha-de-Lossada, C, Mazzotta, C, Gabrielli, F, Papa, F T, Gómez-Huertas, C, García-López, C, Urbinati, F, Rachwani-Anil, R, García-Lorente, M, Sánchez-González, J M, Rechichi, M, Rubegni, G & Borroni, D 2023, 'Ocular Surface Microbiota in Naïve Keratoconus : A Multicenter Validation Study', Journal of clinical medicine, vol. 12, no. 19, 6354. https://doi.org/10.3390/jcm12196354
dc.identifier.doi10.3390/jcm12196354
dc.identifier.issn2077-0383
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/16998
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85173828520&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofJournal of clinical medicine
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectkeratoconus
dc.subjectmetagenomics
dc.subjectmicrobiota
dc.subjectvalidation-study
dc.subject3.2 Clinical medicine
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectGeneral Medicine
dc.titleOcular Surface Microbiota in Naïve Keratoconus : A Multicenter Validation Studyen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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