Characterization of the transporterB0AT3 (Slc6a17) in the rodent central nervous system
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Date
2013-05-14
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Abstract
Background The vesicular B0AT3 transporter (SLC6A17), one of the members of the SLC6 family, is a transporter for neutral amino acids and is exclusively expressed in brain. Here we provide a comprehensive expression profile of B0AT3 in mouse brain using in situ hybridization and immunohistochemistry. Results We confirmed previous expression data from rat brain and used a novel custom made antibody to obtain detailed co-labelling with several cell type specific markers. B0AT3 was highly expressed in both inhibitory and excitatory neurons. The B0AT3 expression was highly overlapping with those of vesicular glutamate transporter 2 (VGLUT2) and vesicular glutamate transporter 1 (VGLUT1). We also show here that Slc6a17mRNA is up-regulated in animals subjected to short term food deprivation as well as animals treated with the serotonin reuptake inhibitor fluoxetine and the dopamine/noradrenaline reuptake inhibitor bupropion. Conclusions This suggests that the B0AT3 transporter have a role in regulation of monoaminergic as well as glutamatergic synapses.
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3.2 Clinical medicine, 1.4. Reviewed scientific article published in Latvia or abroad in a scientific journal with an editorial board (including university editions)
Citation
Hellsten, S, Hagglund, M, Bagchi, S, Ljungdahl, A, Nilsson, V, Winnergren, S, Stephansson, O, Svirskis, Š, Rumaks, J, Klusa, V, Schioth, H & Fredriksson, R 2013, 'Characterization of the transporterB0AT3 (Slc6a17) in the rodent central nervous system', BMC Neuroscience, vol. 54, no. 14, pp. 1-18.