The link between mitochondrial DNA hypervariable segment I heteroplasmy and ageing among genetically unrelated Latvians
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2011-07
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Abstract
Various studies have demonstrated that mitochondrial DNA (mtDNA) heteroplasmy tends to increase with age and that the observed frequency of heteroplasmy among populations mostly depends on the way it is measured. Therefore, we investigated age-related association on the presence of mtDNA heteroplasmy within the hypervariable segment 1 (HVS-I) in a selected study group. The study group consisted of 300 maternally unrelated Latvians ranging in age from 18 to over 90. years. To determine the optimal method for mtDNA heteroplasmy detection, three approaches were used: (i) SURVEYOR Mutation Detection Kit, (ii) sequencing and (iii) denaturing gradient-gel electrophoresis (DGGE). Among the studied individuals, 30.3% were found to be heteroplasmic. The distribution of heteroplasmy statistically significantly increased with individuals' age (17%; 95% confidence interval [CI] 0.095-0.244 in the 18-40. year age group vs. 39%; [CI] 0.294-0.487 in the > 90 year age group). Heteroplasmy occurred in a total of 21 different positions within HVS-I, and was the most frequent at fast-mutated positions 16189, 16304 and 16311. The results indicate that heteroplasmy in HVS-I is relatively common and occurs in a broad spectrum of sites. The above is supported by evidence to eventual increase of the probability of heteroplasmy with age due to specific mitochondrial haplogroup background.
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Funding Information: This research was supported by a grant from the Latvian Council of Science National Programs no. 09.1284 and 10.0010.01 , and the European Social Fund project no. 1DP/1.1.2.0/09/APIA/VIAA/150 . We wish to express our gratitude to two anonymous reviewers for their helpful suggestions. We acknowledge Ligija Ignatovica for technical assistance in sequencing analysis and the administrators of the Genome Database of the Latvian Population, Latvian Biomedical Research and Study Centre.
Keywords
Ageing, Detection of mtDNA heteroplasmy, Heteroplasmy, Latvians, MtDNA, 1.6 Biological sciences, 1.1. Scientific article indexed in Web of Science and/or Scopus database, Biochemistry, Ageing, Molecular Biology, Genetics, Endocrinology, Cell Biology
Citation
Pliss, L, Brakmanis, A, Ranka, R, Elferts, D, Krumina, A & Baumanis, V 2011, 'The link between mitochondrial DNA hypervariable segment I heteroplasmy and ageing among genetically unrelated Latvians', Experimental Gerontology, vol. 46, no. 7, pp. 560-568. https://doi.org/10.1016/j.exger.2011.02.016