Peripheral Blood Mononuclear Cells' Proliferative Response to Human Parvovirus B19 Antigens in Patients with Rheumatoid Arthritis

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dc.contributor.authorBratslavska, Olga
dc.contributor.authorKozireva, Svetlana
dc.contributor.authorKadisa, Anda
dc.contributor.authorSvirskis, Simons
dc.contributor.authorPavlova, Jeļena
dc.contributor.authorLejnieks, Aivars
dc.contributor.authorMurovska, Modra
dc.contributor.institutionInstitute of Microbiology and Virology
dc.contributor.institutionDepartment of Internal Diseases
dc.date.accessioned2021-05-07T09:45:01Z
dc.date.available2021-05-07T09:45:01Z
dc.date.issued2016-08-01
dc.descriptionAutore J.Pavlova Scopus datubāzē pie raksta nav norādīta, bet žurnāla "Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences" PDF formāta publikācijā ir norādīta. Publisher Copyright: © 2016 by Svetlana Kozireva. Copyright: Copyright 2016 Elsevier B.V., All rights reserved.
dc.description.abstractThis study aimed to determine peripheral blood mononuclear cells' (PBMC) proliferative response to parvovirus B19 (B19) antigens in rheumatoid arthritis (RA) patients and possible changes in proliferative response due to chemotherapy. Serum and blood samples of 52 RA patients and 25 sex and age matched healthy individuals were examined for the presence of anti-B19 IgG and IgM class antibodies and virus specific DNA sequence by the recomLine B19 test and nested polymerase chain reaction, respectively. The PBMC proliferative activity was estimated on the 3rd and 6th day of PBMC cultivation in the presence of virus and B19 VP1/VP2 peptide, using thymidine incorporation assay. On the 3rd day, PBMC response to B19 antigens was detected in 74.1% RA patients with active, in 44.8%-with remote and in 40%-with latent stage of persistent B19 infection, while in the control group the response was observed only in two individuals with active viral infection. On the 6th day, the response was found in 50% RA patients with active, 68.9%-with remote and in 80%-with latent stage of latent persistent infection as well as in 41.1% remotely infected control individuals. The highest PBMC mean stimulation indices were detected in the RA patients and control persons with active infection as well as in RA patients with latent stage of persistent viral infection. On the 3rd and 6th day, strong proliferative response was significantly more frequently observed in RA patients not receiving methotrexate treatment, compared to the patients receiving methotrexate treatment in different combinations with other drugs. RA patients had more frequent and faster response to B19 antigens than apparently healthy persons.en
dc.description.statusPeer reviewed
dc.format.extent7
dc.format.extent900293
dc.identifier.citationBratslavska, O, Kozireva, S, Kadisa, A, Svirskis, S, Pavlova, J, Lejnieks, A & Murovska, M 2016, 'Peripheral Blood Mononuclear Cells' Proliferative Response to Human Parvovirus B19 Antigens in Patients with Rheumatoid Arthritis', Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences, vol. 70, no. 4, pp. 175-181. https://doi.org/10.1515/prolas-2016-0023
dc.identifier.doi10.1515/prolas-2016-0023
dc.identifier.issn1407-009X
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/4125
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=84983395537&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofProceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectautoimmune disease
dc.subjectB19 infection
dc.subjectcellular response to VP1/VP2 B19 peptide
dc.subject3.2 Clinical medicine
dc.subject1.6 Biological sciences
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectGeneral
dc.titlePeripheral Blood Mononuclear Cells' Proliferative Response to Human Parvovirus B19 Antigens in Patients with Rheumatoid Arthritisen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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