Unlocking the Therapeutic Potential of Freshwater Sapropel Extracts : In Vitro Analysis and Antioxidant Profiling for Skincare Applications

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dc.contributor.authorKļaviņa, Aneka
dc.contributor.authorReste, Jeļena
dc.contributor.authorMārtiņsone, Inese
dc.contributor.authorVanadziņš, Ivars
dc.contributor.authorLece, Anna
dc.contributor.authorPavlovska, Ilona
dc.contributor.institutionDepartment of Occupational and Environmental Medicine
dc.contributor.institutionInstitute of Occupational Safety and Environmental Health
dc.contributor.institutionBioķīmijas zinātniskā laboratorija
dc.date.accessioned2024-05-21T14:00:01Z
dc.date.available2024-05-21T14:00:01Z
dc.date.issued2024-03-27
dc.descriptionPublisher Copyright: © 2024 by the authors.
dc.description.abstractBackground and Objective: Sapropel, a biologically active sedimentary deposit, is high in organic matter and minerals and has been shown to offer health benefits. Its constituents, humic acid (HA) and fulvic acid (FA), have been found to have some therapeutic applications. The aim of this study was to determine the potential therapeutically significant properties of freshwater sapropel extracts: their polyacid content, antioxidative (AO) status, and biological activity in cell culture. Materials and Methods: Freshwater lakes from the southeast region of Latvia were investigated layer by layer. The total organic carbon (TOC) was determined through combustion using the catalytic oxidation method, HA and FA were measured via acid perspiration, and the total polyphenol content (TPC) and total antioxidant status (TAS) was analysed spectrophotometrically. Sapropel extracts’ regenerative abilities were tested in vitro using a Cell-IQ real-time monitoring system on mouse BALB/c 3T3 fibroblasts and human keratinocyte HaCaT cell lines. Cytotoxicity was measured through neutral red uptake assessment as a concentration-dependent reduction in the uptake of neutral red dye relative to a vehicle control or untreated cells. Results: The highest AO activity was observed in sapropel extracts with elevated concentrations of HA and TPC from Audzelu Lake (1.08 ± 0.03 mmol/L), and the lowest activity was found in extracts from Ivusku Lake (0.31 ± 0.01 mmol/L). Correspondingly, the concentrations of HA in Audzelu and Ivusku Lakes were recorded as 45.2 and 27.4 mg/g, respectively. High concentrations of HA promoted in vitro cell growth upon short-term exposure (up to 6 h). Conclusions: The results show that high TPC correlates with AO status and sapropel extracts with higher concentrations of HA exhibit greater AO activity and promote in vitro cell growth, suggesting a perspective use for short-term topical therapeutic skin applications. However, higher concentrations over longer durations showed cytotoxic effects, indicating the need for further investigation.en
dc.description.statusPeer reviewed
dc.format.extent16
dc.format.extent3244409
dc.identifier.citationKļaviņa, A, Reste, J, Mārtiņsone, I, Vanadziņš, I, Lece, A & Pavlovska, I 2024, 'Unlocking the Therapeutic Potential of Freshwater Sapropel Extracts : In Vitro Analysis and Antioxidant Profiling for Skincare Applications', Medicina (Kaunas), vol. 60, no. 4, 546, pp. 1 -16. https://doi.org/10.3390/medicina60040546
dc.identifier.doi10.3390/medicina60040546
dc.identifier.issn1648-9144
dc.identifier.otherunpaywall: 10.3390/medicina60040546
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/15498
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85191333546&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofMedicina (Kaunas)
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectcell growth
dc.subjecthumic acid
dc.subjectfreshwater sapropel
dc.subjectfulvic acid
dc.subjectin vitro
dc.subjectbiological polyacids
dc.subjectantioxidants
dc.subject1.6 Biological sciences
dc.subject1.4 Chemical sciences
dc.subject3.1 Basic medicine
dc.subject3.4 Medical biotechnology
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectGeneral Medicine
dc.subjectSDG 3 - Good Health and Well-being
dc.titleUnlocking the Therapeutic Potential of Freshwater Sapropel Extracts : In Vitro Analysis and Antioxidant Profiling for Skincare Applicationsen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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