Oral Microbiome Traits of Type 1 Diabetes and Phenylketonuria Patients in Latvia

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dc.contributor.authorAbola, Iveta
dc.contributor.authorGudra, Dita
dc.contributor.authorUstinova, Maija
dc.contributor.authorFridmanis, Davids
dc.contributor.authorEmulina, Darta Elizabete
dc.contributor.authorSkadins, Ingus
dc.contributor.authorBrinkmane, Anda
dc.contributor.authorLauga-Tunina, Una
dc.contributor.authorGailite, Linda
dc.contributor.authorAuzenbaha, Madara
dc.contributor.institutionScientific Laboratory of Molecular Genetics
dc.contributor.institutionDepartment of Conservative Dentistry and Oral Health
dc.contributor.institutionDepartment of Biology and Microbiology
dc.date.accessioned2023-07-29T07:35:01Z
dc.date.available2023-07-29T07:35:01Z
dc.date.issued2023-05-31
dc.descriptionFunding Information: The study was funded by Riga Stradins University Internal scientific budgets. Publisher Copyright: © 2023 by the authors.
dc.description.abstractSome metabolic disorder treatments require patients to follow a specific diet or to consume supplements that, over time, can lead to oral microbiome alterations. Well-known disorders requiring such treatment are phenylketonuria (PKU), an inborn error of amino acid metabolism, and type 1 diabetes (T1D), a metabolic disorder that requires a specific diet regimen. Therefore, the aim of this study was to investigate the oral health and microbiome characteristics that might contribute to caries activity and periodontal disease risk in PKU and T1D patients. In this cross-sectional study, 45 PKU patients, 24 T1D patients, and 61 healthy individuals between the ages of 12 and 53 years were examined. Their anamnestic data and dental status were assessed by one dentist. Microbial communities were detected from saliva-isolated DNA using 16S rRNA gene V3–V4 sequencing on Illumina MiSeq sequencing platform. Results revealed that the PKU patient group displayed the highest number of extracted teeth (on average 1.34), carious teeth (on average 4.95), and carious activity (44.44% of individuals) compared to the T1D and CTRL groups. The lowest numbers of filled teeth (on average 5.33) and extracted teeth (on average 0.63) per individual were observed in T1D patients. Gingivitis appeared more often in the T1D group; however, possible risk of periodontal disease was seen in both the T1D and PKU patient groups. The highest number of differentially abundant genera was detected in the PKU group (n = 20), with enrichment of Actinomyces (padj = 4.17 × 10−22), Capnocytophaga (padj = 8.53 × 10−8), and Porphyromonas (padj = 1.18 × 10−5) compared to the CTRL group. In conclusion, the dental and periodontal health of PKU patients was found to be significantly inferior compared to T1D patients and healthy controls. T1D patients showed early signs of periodontal disease. Several genera that correlate with periodontal disease development were found in both groups, thus suggesting that T1D and PKU patients should seek early and regular dental advice and be educated about proper oral hygiene practices.en
dc.description.statusPeer reviewed
dc.format.extent19
dc.format.extent4159974
dc.identifier.citationAbola, I, Gudra, D, Ustinova, M, Fridmanis, D, Emulina, D E, Skadins, I, Brinkmane, A, Lauga-Tunina, U, Gailite, L & Auzenbaha, M 2023, 'Oral Microbiome Traits of Type 1 Diabetes and Phenylketonuria Patients in Latvia', Microorganisms, vol. 11, no. 6, 1471. https://doi.org/10.3390/microorganisms11061471
dc.identifier.doi10.3390/microorganisms11061471
dc.identifier.otherunpaywall: 10.3390/microorganisms11061471
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/14178
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85163861939&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofMicroorganisms
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subject16S rRNA sequencing
dc.subjectmicrobiome
dc.subjectphenylketonuria
dc.subjecttype 1 diabetes
dc.subject1.6 Biological sciences
dc.subject3.2 Clinical medicine
dc.subject3.1 Basic medicine
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectMicrobiology
dc.subjectMicrobiology (medical)
dc.subjectVirology
dc.subjectSDG 3 - Good Health and Well-being
dc.titleOral Microbiome Traits of Type 1 Diabetes and Phenylketonuria Patients in Latviaen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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