Analysis of Inflammatory and Regulatory Cytokines in the Milk of Dairy Cows with Mastitis : A Comparative Study with Healthy Animals

Simple item page
dc.contributor.authorLohova, Elizabete
dc.contributor.authorPilmane, Māra
dc.contributor.authorŠerstņova, Ksenija
dc.contributor.authorMelderis, Ivars
dc.contributor.authorGontar, Lukasz
dc.contributor.authorKochanski, Maksymilian
dc.contributor.authorDrutowska, Andzelika
dc.contributor.authorGergely, Maroti
dc.contributor.authorPrieto Simón, Beatriz
dc.contributor.institutionInstitute of Anatomy and Anthropology
dc.date.accessioned2024-11-22T13:15:01Z
dc.date.available2024-11-22T13:15:01Z
dc.date.issued2024
dc.descriptionPublisher Copyright: © 2024 The Author(s). Published with license by Taylor & Francis Group, LLC.
dc.description.abstractBovine mastitis remains a major problem in the global dairy cattle industry. The acute invasion of udder by pathogens induces innate immune response as the first defence mechanism in subclinical and clinical mastitis. The aim of the study was to determine inflammatory and regulatory cytokines IL-2, IL-4, TGF-β1, IL-17A, beta-defensin 3 and IL-10 and their potential changes in milk of dairy cows with subclinical and clinical mastitis, and to compare the findings with healthy animals. Milk samples from 15 holstein Friesian breed cows were used in the study. Cows were divided into three groups based on their health status (5 healthy, 5 subclinical and 5 clinical animals). All samples were tested using immunohistochemistry to evaluate IL-2, IL-4, IL-10, IL17A, TGF-β1 and β-Def 3 proteins. Expression of all proteins was detected in all milk samples. High expression of IL-2, IL-4, IL17A, TGF-β1 was detected in healthy cows’ milk and in milk of cows with subclinical and clinical mastitis. However, expression of IL-10 and β-Def 3 in milk samples of healthy cows was significantly higher compared to the milk of cows with subclinical and clinical mastitis (p <.001). IL-10 and β-Def 3 can be considered as informative biomarkers in diagnosis of subclinical and clinical mastitis.en
dc.description.statusPeer reviewed
dc.format.extent25
dc.format.extent1968866
dc.identifier.citationLohova, E, Pilmane, M, Šerstņova, K, Melderis, I, Gontar, L, Kochanski, M, Drutowska, A, Gergely, M & Prieto Simón, B 2024, 'Analysis of Inflammatory and Regulatory Cytokines in the Milk of Dairy Cows with Mastitis : A Comparative Study with Healthy Animals', Immunological Investigations, vol. 53, no. 8, pp. 1397-1421. https://doi.org/10.1080/08820139.2024.2404623
dc.identifier.doi10.1080/08820139.2024.2404623
dc.identifier.issn0882-0139
dc.identifier.otherMendeley: 05bc61f3-4e07-3094-90f8-e44d08966a2d
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/16914
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85204067807&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofImmunological Investigations
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectcytokines
dc.subjectinflammatory markers
dc.subjectmastitis
dc.subjectbovine milk
dc.subjectinflammation
dc.subject3.1 Basic medicine
dc.subject4.3 Veterinary science
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.titleAnalysis of Inflammatory and Regulatory Cytokines in the Milk of Dairy Cows with Mastitis : A Comparative Study with Healthy Animalsen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Analysis_of_Inflammatory_and_Regulatory_Cytokines.pdf
Size:
1.88 MB
Format:
Adobe Portable Document Format
Download

Collections

Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure