Growth factors, their receptors, neuropeptide-containing innervation, and matrix metalloproteinases in the proximal and distal ends of the esophagus in children with esophageal atresia

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dc.contributor.authorPilmane, Mara
dc.contributor.authorOzoliņa, Linda
dc.contributor.authorĀbola, Zane
dc.contributor.authorPetersons, Aigars
dc.contributor.authorPopkovs, Vjačeslavs
dc.contributor.authorDabužinskiene, Anita
dc.contributor.authorVetra, Janis
dc.contributor.institutionInstitute of Anatomy and Anthropology
dc.date.accessioned2021-04-28T10:05:01Z
dc.date.available2021-04-28T10:05:01Z
dc.date.issued2011
dc.description.abstractObjective: The pathogenesis of esophageal atresia (EA) remains unknown despite a relatively high incidence of this anomaly in population affecting 1 newborn per 3000 live births. The aim of this study was to examine the relative occurrence of growth factors, their receptors, neuropeptide-containing innervation, and tissue-degradating enzymes - matrix metalloproteinases - in the proximal and distal parts of the esophagus with EA. Materials and Methods: A histopathological study was conducted on 15 patients with EA. Tissues were processed for NGFRp75, PGP 9.5, TGF-β, FGFR, VEGF, EGFR and MMP-2 by means of biotin-streptavidin immunohistochemistry. Results: In the control and EA-affected distal esophageal specimens, numerous and abundant NGFR-containing structures were detected, while in the proximal part of the esophagus, a decrease in their number was observed in patients. PGP 9.5 also marked neuronal structures similarly. TGF-β was found only in occasional cells in the EA-affected esophageal specimens, while control material demonstrated moderate to numerous TGF-β-containing structures. Abundance of FGFR and only occasional appearance of VEGF-positive cells were found in both the control and EA-affected material. A moderate number of connective tissue cells in controls contained EGFR. Compared with controls, the number of MMP-2 expressing cells in the EA-affected tissues was decreased in the proximal esophagus. Conclusions: A decrease in PGP 9.5-containing neuronal structures in the proximal esophagus supports insufficient innervation of this part of the organ in EA. A decrease in MMP-2 positive cells in the esophageal atresia-affected proximal esophagus indicates also a possible decrease of tissue adaptive and regenerative reactions. Low expression of TGF-β and almost the absence of EGFR in the EA-affected specimens may result in disturbances of cell growth, proliferation, and differentiation, indicating a significant role of these substances in morphopathogenesis of EA. FGFR and VEGF seem not to characterize EA pathogenesis.en
dc.description.statusPeer reviewed
dc.format.extent8
dc.format.extent807006
dc.identifier.citationPilmane, M, Ozoliņa, L, Ābola, Z, Petersons, A, Popkovs, V, Dabužinskiene, A & Vetra, J 2011, 'Growth factors, their receptors, neuropeptide-containing innervation, and matrix metalloproteinases in the proximal and distal ends of the esophagus in children with esophageal atresia', Medicina, vol. 47, no. 8, pp. 453-460. https://doi.org/10.3390/medicina47080066
dc.identifier.doi10.3390/medicina47080066
dc.identifier.issn1010-660X
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/3969
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=84856647840&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofMedicina
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAtresia
dc.subjectChildren
dc.subjectEsophagus
dc.subjectGrowth factors
dc.subjectImmunohistochemistry
dc.subject3.1 Basic medicine
dc.subject3.2 Clinical medicine
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectGeneral Medicine
dc.titleGrowth factors, their receptors, neuropeptide-containing innervation, and matrix metalloproteinases in the proximal and distal ends of the esophagus in children with esophageal atresiaen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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