Who Could Be Blamed in the Case of Discrepant Histology and Serology Results for Helicobacter pylori Detection?

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dc.contributor.authorSkrebinska, Sabine
dc.contributor.authorMegraud, Francis
dc.contributor.authorDaugule, Ilva
dc.contributor.authorSantare, Daiga
dc.contributor.authorIsajevs, Sergejs
dc.contributor.authorLiepniece-Karele, Inta
dc.contributor.authorBogdanova, Inga
dc.contributor.authorRudzite, Dace
dc.contributor.authorVangravs, Reinis
dc.contributor.authorKikuste, Ilze
dc.contributor.authorVanags, Aigars
dc.contributor.authorTolmanis, Ivars
dc.contributor.authorSavcenko, Selga
dc.contributor.authorAlix, Chloé
dc.contributor.authorHerrero, Rolando
dc.contributor.authorPark, Jin Young
dc.contributor.authorLeja, Marcis
dc.date.accessioned2022-02-01T09:50:01Z
dc.date.available2022-02-01T09:50:01Z
dc.date.issued2022-01
dc.descriptionFunding Information: Acknowledgments: The study was funded in part by the Fundamental and Applied Research Project Program in Latvia, project No. lzp-2018/1-0135 “Research on implementation of a set of measures for prevention of gastric cancer mortality by eradication H. pylori and timely recognition of precancerous lesions”. We acknowledge the entire GISTAR study team as well as the infrastructure provided by the Digestive Diseases Centre GASTRO for endoscopy and the Academic Histology Laboratory for pathology infrastructures. Our acknowledgements also to Biohit Oyj, Finland, for their support with laboratory reagents. Funding Information: Funding: The work is funded by ERDF (European Regional Development Fund) within the frame-work of 2nd part of measure 1.1.1.1. ‘Practical Studies’, project ID Nr. 1.1.1.1/18/A/184. Funding Information: The work is funded by ERDF (European Regional Development Fund) within the framework of 2nd part of measure 1.1.1.1. ?Practical Studies?, project ID Nr. 1.1.1.1/18/A/184. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
dc.description.abstractBackground. Discrepancies between histology and serology results for Helicobacter pylori detection could be caused by a variety of factors, including a biopsy sampling error, expertise of the pathologist, natural loss of infection due to advanced atrophy, or a false-positive serology in the case of a previous infection, since antibodies may be present in blood following recovery from the infection. Aims. To identify true H. pylori-positive individuals in discrepant cases by serology and histology using real time polymerase chain reaction (RT-PCR) as a gold standard. Methods. Study subjects with discrepant histology and serology results were selected from the GISTAR pilot study data base in Latvia. Subjects having received previous H. pylori eradication therapy or reporting use of proton pump inhibitors, antibacterial medications, or bismuth containing drugs one month prior to upper endoscopy were excluded. We compared the discrepant cases to the corresponding results of RT-PCR performed on gastric biopsies. Results. In total, 97 individuals with discrepant results were identified: 81 subjects were serology-positive/histology-negative, while 16 were serologynegative/histology-positive. Among the serology-positive/histology-negative cases, 64/81 (79.0%) were false-positives by serology and, for the majority, inflammation was absent in all biopsies, while, in the serology-negative/histology-positive group, only 6.2% were proven false-positives by histology. Conclusions. Among this high H. pylori prevalent, middle-aged population, the majority of discrepant cases between serology and histology were due to false positive-serology, rather than false-negative histology. This confirms the available evidence that the choice of treatment should not be based solely on the serological results, but also after excluding previous, self-reported eradication therapy.en
dc.description.statusPeer reviewed
dc.format.extent428131
dc.identifier.citationSkrebinska, S, Megraud, F, Daugule, I, Santare, D, Isajevs, S, Liepniece-Karele, I, Bogdanova, I, Rudzite, D, Vangravs, R, Kikuste, I, Vanags, A, Tolmanis, I, Savcenko, S, Alix, C, Herrero, R, Park, J Y & Leja, M 2022, 'Who Could Be Blamed in the Case of Discrepant Histology and Serology Results for Helicobacter pylori Detection?', Diagnostics, vol. 12, no. 1, 133. https://doi.org/10.3390/diagnostics12010133
dc.identifier.doi10.3390/diagnostics12010133
dc.identifier.issn2075-4418
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/7398
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85122697371&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofDiagnostics
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectDiscrepant cases
dc.subjectH. pylori
dc.subjectHistology
dc.subjectPolymerase chain reaction
dc.subjectSerology
dc.subject3.1 Basic medicine
dc.subject3.2 Clinical medicine
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectClinical Biochemistry
dc.titleWho Could Be Blamed in the Case of Discrepant Histology and Serology Results for Helicobacter pylori Detection?en
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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