Overexpression of LSH1, a member of an uncharacterised gene family, causes enhanced light regulation of seedling development

Abstract

Light regulates plant growth and development through a network of endogenous factors. By screening Arabidopsis activation-tagged lines, we isolated a dominant mutant (light-dependent short hypocotyls 1-D (Ish1-D)) that showed hypersensitive responses to continuous red (cR), far-red (cFR) and blue (cB) light and cloned the corresponding gene, LSH1. LSH1 encodes a nuclear protein of a novel gene family that has homologues in Arabidopsis and rice. The effects of the Ish1-D mutation were tested in a series of photoreceptor mutant backgrounds. The hypersensitivity to cFR and cB light conferred by Ish1-D was abolished in a phyA null background (phyA-201), and the hypersensitivity to cR and cFR light conferred by Ish1-D was much reduced in the phytochrome- chromophore synthetic mutant, hy1-1 (long hypocotyl 1). These results indicate that LSH1 is functionally dependent on phytochrome to mediate light regulation of seedling development.

Description

Keywords

Activation tagging, Arabidopsis, Light, LSH1, New gene family, Photoreceptors, 1.6 Biological sciences, 1.1. Scientific article indexed in Web of Science and/or Scopus database, Genetics, Plant Science, Cell Biology

Citation

Zhao, L, Nakazawa, M, Takase, T, Manabe, K, Kobayashi, M, Seki, M, Shinozaki, K & Matsui, M 2004, 'Overexpression of LSH1, a member of an uncharacterised gene family, causes enhanced light regulation of seedling development', Plant Journal, vol. 37, no. 5, pp. 694-706. https://doi.org/10.1111/j.1365-313X.2003.01993.x