Genome wide analysis of circulating miRNAs in growth hormone secreting pituitary neuroendocrine tumor patients’ plasma

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dc.contributor.authorNiedra, Helvijs
dc.contributor.authorPeculis, Raitis
dc.contributor.authorLitvina, Helena Daiga
dc.contributor.authorMegnis, Kaspars
dc.contributor.authorMadrika, Ilona
dc.contributor.authorBalcere, Inga
dc.contributor.authorRomanovs, Mihails
dc.contributor.authorSteina, Liva
dc.contributor.authorStukens, Janis
dc.contributor.authorBreiksa, Austra
dc.contributor.authorNazarovs, Jurijs
dc.contributor.authorSokolovska, Jelizaveta
dc.contributor.authorLiutkeviciene, Rasa
dc.contributor.authorVilkevicute, Alvita
dc.contributor.authorKonrade, Ilze
dc.contributor.authorRovite, Vita
dc.contributor.institutionDepartment of Internal Diseases
dc.date.accessioned2023-02-24T16:15:01Z
dc.date.available2023-02-24T16:15:01Z
dc.date.issued2022-09-09
dc.descriptionFunding Information: The financial support given by NSFC with Grant No. 11874034, Taishan Scholar Program of Shandong No. ts201712054, and Shandong Science and Technology Enterprise Innovation Capacity Enhancement Project (2021TSGC1036) are highly appreciated for this research. Publisher Copyright: Copyright © 2022 Niedra, Peculis, Litvina, Megnis, Madrika, Balcere, Romanovs, Steina, Stukens, Breiksa, Nazarovs, Sokolovska, Liutkeviciene, Vilkevicute, Konrade and Rovite.
dc.description.abstractBackground: Circulating plasma miRNAs have been increasingly studied in the field of pituitary neuroendocrine tumor (PitNET) research. Our aim was to discover circulating plasma miRNAs species associated with growth hormone (GH) secreting PitNETs versus assess how the plasma levels of discovered miRNA candidates are impacted by SSA therapy and whether there is a difference in their levels between GH secreting PitNETs versus other PitNET types and healthy individuals. Design: We compared plasma miRNA content and levels before and after surgery focusing on GH secreting PitNET patients. Selected miRNA candidates from our data and literature were then tested in a longitudinal manner in somatostatin analogues (SSA) treatment group. Additionally, we validated selected targets in an independent GH secreting PitNET group. Methods: miRNA candidates were discovered using the whole miRNA sequencing approach and differential expression analysis. Selected miRNAs were then analyzed using real-time polymerase chain reaction (qPCR). Results: Whole miRNA sequencing discovered a total of 16 differentially expressed miRNAs (DEMs) in GH secreting PitNET patients’ plasma 24 hours after surgery and 19 DEMs between GH secreting PitNET patients’ plasma and non-functioning (NF) PitNET patients’ plasma. Seven miRNAs were selected for further testing of which miR-625-5p, miR-503-5p miR-181a-2-3p and miR-130b-3p showed a significant downregulation in plasma after 1 month of SSA treatment. mir-625-5p was found to be significantly downregulated in plasma of GH secreting PitNET patients vs. NF PitNET patients. miR-625-5p alongside miR-130b-3p were also found to be downregulated in GH PitNETs compared to healthy individuals. Conclusions: Our study suggests that expression of plasma miRNAs miR-625-5p, miR-503-5p miR-181a-2-3p and miR-130b-3p in GH secreting PitNETs is affected by SSA treatment. Additionally, miR-625-5p can distinguish GH secreting PitNETs from other PitNET types and healthy controls warranting further research on these miRNAs for treatment efficacy.en
dc.description.statusPeer reviewed
dc.format.extent1552270
dc.identifier.citationNiedra, H, Peculis, R, Litvina, H D, Megnis, K, Madrika, I, Balcere, I, Romanovs, M, Steina, L, Stukens, J, Breiksa, A, Nazarovs, J, Sokolovska, J, Liutkeviciene, R, Vilkevicute, A, Konrade, I & Rovite, V 2022, 'Genome wide analysis of circulating miRNAs in growth hormone secreting pituitary neuroendocrine tumor patients’ plasma', Frontiers in Oncology, vol. 12, 894317. https://doi.org/10.3389/fonc.2022.894317
dc.identifier.doi10.3389/fonc.2022.894317
dc.identifier.issn2234-943X
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/10639
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85138781002&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofFrontiers in Oncology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectacromegaly
dc.subjectcirculating plasma micro-RNAs
dc.subjectgrowth hormone secreting pituitary neuroendocrine tumor
dc.subjectmicro-RNA differential expression
dc.subjectsomatostatin analogue treatment
dc.subject1.6 Biological sciences
dc.subject3.1 Basic medicine
dc.subject3.2 Clinical medicine
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectOncology
dc.subjectCancer Research
dc.subjectSDG 3 - Good Health and Well-being
dc.titleGenome wide analysis of circulating miRNAs in growth hormone secreting pituitary neuroendocrine tumor patients’ plasmaen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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