Sila virša (Calluna vulgaris) laksta ekstraktu antibakteriālās aktivitātes noteikšana
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Date
2021
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Rīgas Stradiņa universitāte
Rīga Stradiņš University
Rīga Stradiņš University
Abstract
Pieaugošā rezistento baktēriju izplatīšanās ir viena no galvenajām problēmām mūsdienu infekciju slimību medicīnā. Veselības aprūpes organizāciju prioritāte gan visā pasaulē, gan atsevišķās valstīs, ir jaunu antibakteriālo vielu izpēte. Daudzi pētījumi tiek veikti tieši jaunu antibakteriālu vielu izpētē, kas iegūtas no ārstniecības augiem.
Sila virsis ir pundurkrūms ar plašu augšanas areālu. Tas ir atzīts ārstniecības augs, kas plaši tiek pielietots tradicionālajā tautas medicīnā, ar potenciālām antibakteriālām īpašībām.
Šī darba mērķis ir noteikt Calluna vulgaris ekstraktu optimālo antibakteriālo aktivitāti pie dažādiem šķīdinātājiem pie mainīgām to koncentrācijām.
Calluna vulgaris laksta ekstrakti tika šķīdināti etilspirtā un acetonā pie dažādām koncentrācijām – 30%, 50% un 70%. To antibakteriālā aktivitāte tika noteikta pret Staphylococcus aureus (V256), Escherichia coli (V252), Escherichia coli (V4), Staphylococcus aureus (ref.), Escherichia coli (ref.), Streptococcus agalactiae (V171), Serratia liquerfaciens (V251), Streptococcus uberis (V243) baktēriju kultūrām.
Antibakteriālās aktivitātes noteikšanai tika izmantota disku difūzijas metode. Katrai baktēriju kultūrai tikai mērītas inhibīcijas zonas izmēri.
Iegūtie dati apstrādāti ar IBM SPSS un Microsoft Excel 2016 programmatūrām. Tika pielietotas aprakstošās statistikas un korelāciju analīžu metodes.
Iegūtie rezultāti parādīja, ka visaugstākā antibakteriālā aktivitāte novērojama Calluna vulgaris ekstraktiem ekstrahētiem 30% etilspirta un acetona šķīdumos. Palielinoties šķīdinātāja koncentrācijai, inhibīcijas zona samazinājās. Ar visaugstāko antibakteriālo aktivitāti starp visām baktēriju kultūrām izcēlās Staphylococcus aureus (V256), kura ekstrahēšanai tika izmantots 30% etilspirta šķīdums.
Tika secināts, ka optimālo antibakteriālo iedarbību vislabāk parādīja ekstrakts šķīdināts 30% etanola šķīdumā. Pie augstākas koncentrācijas šķīdinātājiem tika reģistrēta mazāka antibakteriālā aktivitāte. Calluna vulgaris laksta ekstrakts pierādīja savu antibakteriālo īpašību potenciālu pret visām pētījumā izmantotajām baktēriju kultūrām.
Calluna vulgaris laksta ekstrakta antibakteriālās īpašības nākotnē varētu tikt pielietotas pētījumā apskatīto baktēriju kultūru infekciju slimību ārstēšanā.
The rising number of drug resistant and multi-drug resistant bacterial strains is one of the key issues of present day infectious disease medicine. The research for new antimicrobial substances to counteract this problem is a priority for health organisations globally and in individual countries. A lot of new studies are devoted to exploring new antibacterial substances from medical plants that could serve as alternative against bacterial diseases. Calluna vulgaris is dwarf bush with wide growth area. It is recorgnised as medical plant widely used in folk traditional medicine, also with potential antibacterial properties. The aim of this study is to determine the optimal antibacterial activity of Calluna vulgaris extracts at different solvents at varying concentrations. Extracts of Calluna vulgaris were diluted with acetone and ethanol solutions in varying concentrations – 30%, 50% and 70%. Diluted extracts were tested for their antibacterial activities against Staphylococcus aureus (V256), Escherichia coli (V252), Escherichia coli (V4), Staphylococcus aureus (ATCC), Escherichia coli (ATCC), Streptococcus agalactiae (V171), Serratia liquerfaciens (V251), Streptococcus uberis (V243). The antibacterial activity of the plant extracts was evaluated using a disk diffusion assay. Inhibition zones for each bacteria were measured. The obtained data were statistically processed employing the IBM SPSS and Microsoft Excel 2016 software using descriptive statistics and correlation analysis. Results showed that the highest antibacterial activity was for 30% ethanol and acetone solutions. The higher concentration was used to determine it, the smaller inhibition zone was observed. Highest antibacterial activity amongst all bacteria showed Staphylococcus aureus (V256), exposed to extract diluted with 30% ethanol solution. Highest antibacterial activity looking at all bacterial cultures together showed Calluna culgaris extract diluted with 30% ethanol solution. From the concentrations used in the experiment as most optimal was observed 30% in ethanol solution. The higher the concentration of solutions were used, the less antibacterial activity was observed. Calluna vulgaris sterm extract diluted in both solutions showed promising potential of antibacterial activities against all tested bacterial cultures. Calluna vulgaris antibacterial properties in future could be used in infectious disease treatment of the studied bacterial cultures.
The rising number of drug resistant and multi-drug resistant bacterial strains is one of the key issues of present day infectious disease medicine. The research for new antimicrobial substances to counteract this problem is a priority for health organisations globally and in individual countries. A lot of new studies are devoted to exploring new antibacterial substances from medical plants that could serve as alternative against bacterial diseases. Calluna vulgaris is dwarf bush with wide growth area. It is recorgnised as medical plant widely used in folk traditional medicine, also with potential antibacterial properties. The aim of this study is to determine the optimal antibacterial activity of Calluna vulgaris extracts at different solvents at varying concentrations. Extracts of Calluna vulgaris were diluted with acetone and ethanol solutions in varying concentrations – 30%, 50% and 70%. Diluted extracts were tested for their antibacterial activities against Staphylococcus aureus (V256), Escherichia coli (V252), Escherichia coli (V4), Staphylococcus aureus (ATCC), Escherichia coli (ATCC), Streptococcus agalactiae (V171), Serratia liquerfaciens (V251), Streptococcus uberis (V243). The antibacterial activity of the plant extracts was evaluated using a disk diffusion assay. Inhibition zones for each bacteria were measured. The obtained data were statistically processed employing the IBM SPSS and Microsoft Excel 2016 software using descriptive statistics and correlation analysis. Results showed that the highest antibacterial activity was for 30% ethanol and acetone solutions. The higher concentration was used to determine it, the smaller inhibition zone was observed. Highest antibacterial activity amongst all bacteria showed Staphylococcus aureus (V256), exposed to extract diluted with 30% ethanol solution. Highest antibacterial activity looking at all bacterial cultures together showed Calluna culgaris extract diluted with 30% ethanol solution. From the concentrations used in the experiment as most optimal was observed 30% in ethanol solution. The higher the concentration of solutions were used, the less antibacterial activity was observed. Calluna vulgaris sterm extract diluted in both solutions showed promising potential of antibacterial activities against all tested bacterial cultures. Calluna vulgaris antibacterial properties in future could be used in infectious disease treatment of the studied bacterial cultures.
Description
Pediatrija
Pediatrics
Veselības aprūpe
Health Care
Pediatrics
Veselības aprūpe
Health Care
Keywords
Virši, ekstrakts, antibakteriālā aktivitāte, Heather, antibacterial activity