Uspostavljanje referentnih intervala za multimere faktora von Willebrand

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dc.contributor.authorPikta, Marika
dc.contributor.authorVasse, Marc
dc.contributor.authorSmock, Kristi J.
dc.contributor.authorMoser, Karen A.
dc.contributor.authorvan Dievoet, Marie Astrid
dc.contributor.authorLejniece, Sandra
dc.contributor.authorSzanto, Timea
dc.contributor.authorBautista, Hector
dc.contributor.authorNouadje, George
dc.contributor.authorBanys, Valdas
dc.contributor.institutionRīga Stradiņš University
dc.date.accessioned2022-08-16T21:03:10Z
dc.date.available2022-08-16T21:03:10Z
dc.date.issued2022
dc.descriptionFunding Information: We are grateful to Sebia (France) for the funding of this project. We would like to thank all the medical/laboratory staff and the volunteers who contributed to this study. The preliminary results of this study were discussed at IFCC C-RIDL (Scientific Committee of IFCC on Reference Intervals and Decision Limits) closed meeting during Euromedlab2019 Barcelona congress and presented at the ISTH 2020 Virtual Congress (abstract/e-poster). Publisher Copyright: © 2022 Sciendo. All rights reserved.
dc.description.abstractBackground: von Willebrand factor (VWF) multimers (VWF:MM) methodologies are technically difficult, laborious, time consuming, non-standardized and results vary between laboratories. A new semi automated VWF:MM assay is available for routine use (Sebia). Due to lack of reference values for VWF:MM fractions, results interpretation can be challenging in some cases. The aim of this study was to determine reference intervals for low molecular weight (LMWM), intermediate molecular weight (IMWM) and high molecular weight (HMWM) multimers. Methods: By the international cooperation initiated between 4 countries (Estonia, Latvia, France, and USA) 131 samples of relatively healthy individuals were analyzed for VWF:MM (in total 51 males and 80 non-pregnant females aged 17-69 years). Reference intervals were calculated according to CLSI C28-A3 standard. Results: The proposed reference intervals for VWF:MM were calculated for LMWM 10.4-22.5%, IMWM 22.6-37.6%, HMWM 45.6-66.6%. Age related differences were seen in IMWM and HMWM (p<0.001 and 0.038). There was no gender related difference observed. Geographically LMWM results of France were different from the other regions (p<0.05). Conclusions: Quantification of VWF:MM fractions, in addition to qualitative assessment of VWF:MM patterns, has the potential to aid in differential diagnosis of von Willebrand disease (VWD) subtypes. The reference values calculated in this study can be used in future research to establish clinical decision limits.en
dc.description.statusPeer reviewed
dc.format.extent7
dc.format.extent750926
dc.identifier.citationPikta, M, Vasse, M, Smock, K J, Moser, K A, van Dievoet, M A, Lejniece, S, Szanto, T, Bautista, H, Nouadje, G & Banys, V 2022, 'Uspostavljanje referentnih intervala za multimere faktora von Willebrand', Journal of Medical Biochemistry, vol. 41, no. 1, pp. 115-121. https://doi.org/10.5937/JOMB0-31941
dc.identifier.doi10.5937/JOMB0-31941
dc.identifier.issn1452-8258
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/9417
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85124964840&partnerID=8YFLogxK
dc.language.isobos
dc.relation.ispartofJournal of Medical Biochemistry
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectQuantitative analysis
dc.subjectReference intervals
dc.subjectVon Willebrand factor
dc.subjectVon Willebrand factor multimers
dc.subject3.1 Basic medicine
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectClinical Biochemistry
dc.subjectBiochemistry, medical
dc.titleUspostavljanje referentnih intervala za multimere faktora von Willebrandbs
dc.title.alternativeEstablishing reference intervals for von Willebrand factor multimersen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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