Evaluation of PGP 9.5, NGFR, TGFβ1, FGFR1, MMP-2, AT2R2, SHH, and TUNEL in Primary Obstructive Megaureter Tissue

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dc.contributor.authorJunga, Anna
dc.contributor.authorSiņicins, Ivo
dc.contributor.authorPētersons, Aigars
dc.contributor.authorPilmane, Māra
dc.contributor.institutionInstitute of Anatomy and Anthropology
dc.contributor.institutionDepartment of Paediatric Surgery
dc.date.accessioned2024-09-21T19:25:01Z
dc.date.available2024-09-21T19:25:01Z
dc.date.issued2022-02
dc.descriptionFunding Information: Our greatest thanks to Assoc. Prof. Jānis Dobelis and Dr Ainārs Ģīlis for providing the study specimen. The author(s) received no financial support for the research, authorship, and/or publication of this article. Publisher Copyright: © The Author(s) 2021.
dc.description.abstractPrimary obstructive megaureter (POM) morphogenesis is not fully known. The aim of the study was to evaluate the appearance of different factors that might take part in the pathogenesis of POM. Megaureter tissues of 14 children were stained with hematoxylin and eosin as well as with immunohistochemistry for protein gene product 9.5, nerve growth factor receptor, transforming growth factor beta 1 (TGFβ1), fibroblast growth factor receptor 1 (FGFR1), matrix metalloproteinase 2 (MMP-2), angiotensin 2 receptor type 2, and sonic hedgehog (SHH) protein. Apoptosis was detected by terminal dUTP nick-end labeling reaction. POM tissues revealed transitional epithelium with scattered vacuolization, submucosa with inflammatory cells, and focally vacuolized and chaotically organized muscle layers. Apoptosis, appearance of MMP-2, FGFR1, and SHH prevailed, but TGFβ1 positive cell number was lower in patients. Correlation between MMP-2 in epithelium and endothelium, FGFR1 and MMP-2 in epithelium, and TGFβ1 in epithelium and connective tissue in patients was detected. POM morphopathogenesis involves an apoptotic cell death of epithelium and smooth muscle as well as tissue degradation in epithelium and connective tissue of the ureter wall. The decrease of tissue growth through diminished TGFβ1 expression and stimulation of FGFR1 and MMP-2 suggests a disbalance of tissue remodelation in the megaureter wall.en
dc.description.statusPeer reviewed
dc.format.extent11
dc.format.extent1675064
dc.identifier.citationJunga, A, Siņicins, I, Pētersons, A & Pilmane, M 2022, 'Evaluation of PGP 9.5, NGFR, TGFβ1, FGFR1, MMP-2, AT2R2, SHH, and TUNEL in Primary Obstructive Megaureter Tissue', Journal of Histochemistry and Cytochemistry, vol. 70, no. 2, pp. 139-149. https://doi.org/10.1369/00221554211063515
dc.identifier.doi10.1369/00221554211063515
dc.identifier.issn0022-1554
dc.identifier.otherMendeley: 6c734d73-b765-3c3e-bb68-452a9cd5a4fe
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/16698
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85121743287&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofJournal of Histochemistry and Cytochemistry
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectatrophy
dc.subjectdevelopment
dc.subjectgrowth factors
dc.subjectinnervation
dc.subject3.1 Basic medicine
dc.subject3.2 Clinical medicine
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.titleEvaluation of PGP 9.5, NGFR, TGFβ1, FGFR1, MMP-2, AT2R2, SHH, and TUNEL in Primary Obstructive Megaureter Tissueen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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