Investigating ethanol production using the Zymomonas mobilis crude extract

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dc.contributor.authorAminian, Amirhossein
dc.contributor.authorMotamedian, Ehsan
dc.date.accessioned2025-03-11T08:50:01Z
dc.date.available2025-03-11T08:50:01Z
dc.date.issued2023-12
dc.descriptionPublisher Copyright: © 2023, The Author(s).
dc.description.abstractCell-free systems have become valuable investigating tools for metabolic engineering research due to their easy access to metabolism without the interference of the membrane. Therefore, we applied Zymomonas mobilis cell-free system to investigate whether ethanol production is controlled by the genes of the metabolic pathway or is limited by cofactors. Initially, different glucose concentrations were added to the extract to determine the crude extract's capability to produce ethanol. Then, we investigated the genes of the metabolic pathway to find the limiting step in the ethanol production pathway. Next, to identify the bottleneck gene, a systemic approach was applied based on the integration of gene expression data on a cell-free metabolic model. ZMO1696 was determined as the bottleneck gene and an activator for its enzyme was added to the extract to experimentally assess its effect on ethanol production. Then the effect of NAD+ addition at the high concentration of glucose (1 M) was evaluated, which indicates no improvement in efficiency. Finally, the imbalance ratio of ADP/ATP was found as the controlling factor by measuring ATP levels in the extract. Furthermore, sodium gluconate as a carbon source was utilized to investigate the expansion of substrate consumption by the extract. 100% of the maximum theoretical yield was obtained at 0.01 M of sodium gluconate while it cannot be consumed by Z. mobilis. This research demonstrated the challenges and advantages of using Z. mobilis crude extract for overproduction.en
dc.description.statusPeer reviewed
dc.format.extent1085195
dc.identifier.citationAminian, A & Motamedian, E 2023, 'Investigating ethanol production using the Zymomonas mobilis crude extract', Scientific Reports, vol. 13, no. 1, 1165. https://doi.org/10.1038/s41598-023-28396-4
dc.identifier.doi10.1038/s41598-023-28396-4
dc.identifier.issn2045-2322
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/17154
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85146535698&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofScientific Reports
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subject1.6 Biological sciences
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectGeneral
dc.titleInvestigating ethanol production using the Zymomonas mobilis crude extracten
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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