Evaluation of Antimicrobial Resistance in Clinical Isolates of Enterococcus spp. Obtained from Hospital Patients in Latvia

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dc.contributor.authorLabecka, Linda
dc.contributor.authorĶibilds, Juris
dc.contributor.authorCīrulis, Aivars
dc.contributor.authorČeirāne, Evelīna Diāna
dc.contributor.authorZeltiņa, Indra
dc.contributor.authorReinis, Aigars
dc.contributor.authorVilima, Barba
dc.contributor.authorRudzīte, Dace
dc.contributor.authorErts, Renārs
dc.contributor.authorMauliņa, Inga
dc.contributor.authorBandere, Dace
dc.contributor.authorKrūmiņa, Angelika
dc.contributor.institutionDepartment of Infectology
dc.contributor.institutionDepartment of Biology and Microbiology
dc.contributor.institutionDepartment of Pharmaceutical Chemistry
dc.date.accessioned2024-09-02T06:35:02Z
dc.date.available2024-09-02T06:35:02Z
dc.date.issued2024-06
dc.descriptionPublisher Copyright: © 2024 by the authors.
dc.description.abstractBackground and Objective: Enterococci are typically found in a healthy human gastrointestinal tract but can cause severe infections in immunocompromised patients. Such infections are treated with antibiotics. This study addresses the rising concern of antimicrobial resistance (AMR) in Enterococci, focusing on the prevalence of vancomycin-resistant enterococcus (VRE) strains. Materials and Methods: The pilot study involved 140 Enterococci isolates collected between 2021 and 2022 from two multidisciplinary hospitals (with and without local therapeutic drug monitoring protocol of vancomycin) in Latvia. Microbiological assays and whole genome sequencing were used. AMR gene prevalence with resistance profiles were determined and the genetic relationship and outbreak evaluation were made by applying core genome multi-locus sequence typing (cgMLST). Results: The acquired genes and mutations were responsible for resistance against 10 antimicrobial classes, including 25.0% of isolates expressing resistance to vancomycin, predominantly of the vanB type. Genetic diversity among E. faecalis and E. faecium isolates was observed and seven potential outbreak clusters were identified, three of them containing sequence types ST6, ST78 and ST80. The prevalence of vancomycin resistance was highest in the hospital without a therapeutic drug-monitoring protocol and in E. faecium. Notably, a case of linezolid resistance due to a mutation was documented.  Conclusions: The study illustrates the concerning prevalence of multidrug-resistant Enterococci in Latvian hospitals, showcasing the rather widespread occurrence of vancomycin-resistant strains. This highlights the urgency of implementing efficient infection control mechanisms and the need for continuous VRE surveillance in Latvia to define the scope and pattern of the problem, influencing clinical decision making and planning further preventative measures.en
dc.description.statusPeer reviewed
dc.format.extent2107485
dc.identifier.citationLabecka, L, Ķibilds, J, Cīrulis, A, Čeirāne, E D, Zeltiņa, I, Reinis, A, Vilima, B, Rudzīte, D, Erts, R, Mauliņa, I, Bandere, D & Krūmiņa, A 2024, 'Evaluation of Antimicrobial Resistance in Clinical Isolates of Enterococcus spp. Obtained from Hospital Patients in Latvia', Medicina (Lithuania), vol. 60, no. 6, 850. https://doi.org/10.3390/medicina60060850
dc.identifier.doi10.3390/medicina60060850
dc.identifier.issn1010-660X
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/16640
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85197152049&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofMedicina (Lithuania)
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectantimicrobial resistance
dc.subjectenterococci
dc.subjectLRE
dc.subjectresistance genes
dc.subjectvanB
dc.subjectVRE
dc.subject1.6 Biological sciences
dc.subject3.1 Basic medicine
dc.subject3.3 Health sciences
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectGeneral Medicine
dc.subjectSDG 3 - Good Health and Well-being
dc.titleEvaluation of Antimicrobial Resistance in Clinical Isolates of Enterococcus spp. Obtained from Hospital Patients in Latviaen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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