Fenola savienojumu kvantitatīvā satura salīdzinājums parastā biškrēsliņa (Tanacetum vulgare) ziedu drogas etanola un acetona ekstraktos, izmantojot Folin-Ciocalteu metodi
No Thumbnail Available
Date
2020
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Rīgas Stradiņa universitāte
Rīga Stradiņš University
Rīga Stradiņš University
Abstract
Šis pētījums ir daļa no Rīgas Stradiņa universitātes un Latvijas Lauksaimniecības universitātes kopīgā sadarbības projekta, kura ietvaros tiek pētīti no Latvijas florai rakturīgiem fenolsavienojumu saturošiem augiem iegūti ekstrakti, kurus potenciāli var izmantot medicīnā kā jaunus antibakteriālus vai prettārpu līdzekļus.
Mērķis. Noskaidrot, ar kādas koncentrācijas etanola vai acetona šķīdumu (30%, 50% vai 70%) var iegūt visaugstāko fenolsavienojumu kvantitatīvo iznākumu parastā biškrēsliņa (Tanacetum vulgare) ziedu ekstraktā.
Materiāli un metodes. Parastā biškrēsliņa (Tanacetum vulgare) ziedus ievāca un izžāvēja balstoties uz attiecīgām vadlīnijām. Acetona un etanola šķīdumus 3 dažādās koncentrācijās (30%, 50%, 70%) izmantoja, lai izekstraģētu fenolsavienojumus no pētāmā auga ziedu drogas. Ekstraģentus ietvaicēja, izmantojot vakuuma ietvaicētāju. Paraugus sagatavoja analīzēm pēc Folīna-Čikolto metodes. Kopējo fenolsavienojumu koncentrāciju paraugos noteica pēc UV-spektrofotometriskās analīzes, izsakot rezultātus galluskābes ekvivalentos (GSE) uz 100 mg drogas.
Rezultāti. Tika iegūtas šādas kopējās fenolsavienojumu koncentrācijas vidējās vērtības: 70% etanola ekstraktos - 6218,75 mg/100g GSE (SN ±74,77, TI 84,61) , 50% etanola ekstraktos - 6364.58 mg/100g GSE (SN ±264,70, TI 299,54); 30% etanola ekstraktos - 6250.00 mg/100g GSE (SN ±889,83, TI 1006,94); 70% acetona ekstraktos - 7669.17 mg/100g GSE (SN ±413,48, TI 467,90); 50% acetona ekstraktos - 7737.08 mg/100g GSE (SN ±275,25, TI 311,47) ; 30% acetona ekstraktos - 7759.58 mg/100g GSE (SN ±240,97, TI 272,68).
Secinājumi. Ņemot vērā, ka visiem iegūtiem rezultātiem piemīt augsta variabilitāte, nav iespējams uzsvert visefektīvāko ekstraģentu fenolsavienojumu iegūšanai no parastā biškrēsliņa ziedu drogas. Līdz ar to tiek rekomendēts turpināt pētījumu ar lielāku paraugu skaitu katram ekstraģentam.
Research title: COMPARISON OF THE TOTAL PHENOLIC CONTENT IN ETHANOLIC AND ACETONIC COMMON TANSY (Tanacetum vulgare) DRIED FLOWER EXTRACTS USING THE FOLIN-CIOCALTEU METHOD This research is a part of the Rīga Stradiņš University scientific collaboration project with Latvian University of Agriculture that aims to explore extracts gained from phenolics-containing plants typical for Latvian flora that can be used in veterinary medicine as novel antibiotic/anthelminthic agents. Objectives. My research is focused on extraction of phenolic compounds from common tansy flowers with 2 extractants (acetone and ethanol) in 3 concentrations (30%, 50%, 70%) to conclude, which option is the most efficient to use. Materials and Methods. Common tansy (Tanacetum vulgare) flowers have been collected and dried according to the respective guidelines. Acetonic and ethanolic solutions in 3 different concentrations (30%, 50% and 70%) have been used for extraction of phenolic compounds from the dried flowers. The extractants have been removed from extracts via vacuum evaporation. Folin-Ciocalteu method has been used to prepare extracts for the UV-spectroscopy, that then has been performed to estimate the total phenolic content calculated as gallic acid equivalents (GSE) in each of the above-mentioned extracts. Results. Mean results are the following: 70% ethanolic extract contains 6218,75 mg/100g GSE (SD ±74,77, CI 84,61) , 50% ethanolic extract - 6364.58 mg/100g GSE (SD ±264,70, CI 299,54); 30% ethanolic extract - 6250.00 mg/100g GSE (SD ±889,83, CI 1006,94); 70% acetonic extract - 7669.17 mg/100g GSE (SD ±413,48, CI 467,90); 50% acetonic extract - 7737.08 mg/100g GSE (SD ±275,25, CI 311,47) ; 30% acetonic extract - 7759.58 mg/100g GSE (SD ±240,97, CI 272,68). Conclusions. As all acquired results are highly variable, it is not possible to select the most effective extractant for the extraction of phenolic compounds from the common tansy died flowers. It is suggested to continue research by testing more samples of each extractant.
Research title: COMPARISON OF THE TOTAL PHENOLIC CONTENT IN ETHANOLIC AND ACETONIC COMMON TANSY (Tanacetum vulgare) DRIED FLOWER EXTRACTS USING THE FOLIN-CIOCALTEU METHOD This research is a part of the Rīga Stradiņš University scientific collaboration project with Latvian University of Agriculture that aims to explore extracts gained from phenolics-containing plants typical for Latvian flora that can be used in veterinary medicine as novel antibiotic/anthelminthic agents. Objectives. My research is focused on extraction of phenolic compounds from common tansy flowers with 2 extractants (acetone and ethanol) in 3 concentrations (30%, 50%, 70%) to conclude, which option is the most efficient to use. Materials and Methods. Common tansy (Tanacetum vulgare) flowers have been collected and dried according to the respective guidelines. Acetonic and ethanolic solutions in 3 different concentrations (30%, 50% and 70%) have been used for extraction of phenolic compounds from the dried flowers. The extractants have been removed from extracts via vacuum evaporation. Folin-Ciocalteu method has been used to prepare extracts for the UV-spectroscopy, that then has been performed to estimate the total phenolic content calculated as gallic acid equivalents (GSE) in each of the above-mentioned extracts. Results. Mean results are the following: 70% ethanolic extract contains 6218,75 mg/100g GSE (SD ±74,77, CI 84,61) , 50% ethanolic extract - 6364.58 mg/100g GSE (SD ±264,70, CI 299,54); 30% ethanolic extract - 6250.00 mg/100g GSE (SD ±889,83, CI 1006,94); 70% acetonic extract - 7669.17 mg/100g GSE (SD ±413,48, CI 467,90); 50% acetonic extract - 7737.08 mg/100g GSE (SD ±275,25, CI 311,47) ; 30% acetonic extract - 7759.58 mg/100g GSE (SD ±240,97, CI 272,68). Conclusions. As all acquired results are highly variable, it is not possible to select the most effective extractant for the extraction of phenolic compounds from the common tansy died flowers. It is suggested to continue research by testing more samples of each extractant.
Description
Farmācija
Pharmacy
Veselības aprūpe
Health Care
Pharmacy
Veselības aprūpe
Health Care
Keywords
Parastais biškrēsliņš, Tanacetum vulgare, kopējā fenolsavienojumu koncentrācija, Folīna-Čikolto metode, ekstraģentu salīdzinājums., Common tansy, Tanacetum vulgare, total phenolic content, Folin-Ciocalteu method, extractant comparison.