Reconstruction of a regulated two-cell metabolic model to study biohydrogen production in a diazotrophic cyanobacterium Anabaena variabilis ATCC 29413

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dc.contributor.authorShahkouhi, Ali Malek
dc.contributor.authorMotamedian, Ehsan
dc.date.accessioned2025-03-13T11:05:01Z
dc.date.available2025-03-13T11:05:01Z
dc.date.issued2020-01-01
dc.descriptionPublisher Copyright: © 2020 Malek Shahkouhi, Motamedian.
dc.description.abstractAnabaena variabilis is a diazotrophic filamentous cyanobacterium that differentiates to heterocysts and produces hydrogen as a byproduct. Study on metabolic interactions of the two differentiated cells provides a better understanding of its metabolism especially for improving hydrogen production. To this end, a genome-scale metabolic model for Anabaena variabilis ATCC 29413, iAM957, was reconstructed and evaluated in this research. Then, the model and transcriptomic data of the vegetative and heterocyst cells were applied to construct a regulated two-cell metabolic model. The regulated model improved prediction for biomass in high radiation levels. The regulated model predicts that heterocysts provide an oxygen-free environment and then, this model was used to find strategies for improving hydrogen production in heterocysts. The predictions indicate that the removal of uptake hydrogenase improves hydrogen production which is consistent with previous empirical research. Furthermore, the regulated model proposed activation of some reactions to provide redox cofactors which are required for improving hydrogen production up to 60% by bidirectional hydrogenase.en
dc.description.statusPeer reviewed
dc.format.extent1631672
dc.identifier.citationShahkouhi, A M & Motamedian, E 2020, 'Reconstruction of a regulated two-cell metabolic model to study biohydrogen production in a diazotrophic cyanobacterium Anabaena variabilis ATCC 29413', PloS one, vol. 15, no. 1, e0227977. https://doi.org/10.1371/journal.pone.0227977
dc.identifier.doi10.1371/journal.pone.0227977
dc.identifier.issn1932-6203
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/17162
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85078242398&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofPloS one
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subject1.6 Biological sciences
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectGeneral
dc.subjectSDG 7 - Affordable and Clean Energy
dc.titleReconstruction of a regulated two-cell metabolic model to study biohydrogen production in a diazotrophic cyanobacterium Anabaena variabilis ATCC 29413en
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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