Next-generation sequencing for the detection of microorganisms present in human donor corneal preservation medium

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dc.contributor.authorParekh, Mohit
dc.contributor.authorBorroni, Davide
dc.contributor.authorRomano, Vito
dc.contributor.authorKaye, Stephen B.
dc.contributor.authorCamposampiero, Davide
dc.contributor.authorPonzin, DIego
dc.contributor.authorFerrari, Stefano
dc.date.accessioned2025-01-06T10:15:02Z
dc.date.available2025-01-06T10:15:02Z
dc.date.issued2019-04-01
dc.descriptionPublisher Copyright: © Author(s) (or their employer(s)) 2019. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.
dc.description.abstractObjective To detect the presence of microorganisms in the storage media of human donor corneas using next-generation sequencing method. Methods Seven samples from organ culture (OC) group (Cornea Max, Eurobio, Les Ulis, France) with one control (sterile media without any cornea) and seven samples from hypothermic storage group (Cornea Cold, Eurobio) with one control were used for this study. The corneas were placed in the respective storage media for 14 days before collecting the samples. Storage media (2 mL) from each sample were collected in RNAase-free tubes and shipped for ribosomal RNA sequencing of 16 S and 18 S. Simultaneously, another 1 mL of media sample was used for conventional diagnostic method (CDM) using Bactec instruments. Results In both, OC and hypothermic storage and control samples, the most abundant genera were Pseudomonas, Comamonas, Stenotrophomonas, Alcanivorax, Brevundimonas and Nitrobacter. Acidovorax, Acetobacter and Hydrogenophilus were detected mostly in the hypothermic storage group. The most abundant fungal pathogen detected belonged to the genus Malassezia, which was found in both the storage conditions. CDM was negative for microorganisms in all the samples. Conclusion Metagenomics provides full taxonomic profiling of the detected genomic material of the organisms and thus has the potential to deliver a much wider microbiological diagnostic approach than CDM. The costs and turn-around time need to be reduced, and; the detection of viable organisms would help this technology to be introduced into routine clinical practice.en
dc.description.statusPeer reviewed
dc.format.extent1623410
dc.identifier.citationParekh, M, Borroni, D, Romano, V, Kaye, S B, Camposampiero, D, Ponzin, DI & Ferrari, S 2019, 'Next-generation sequencing for the detection of microorganisms present in human donor corneal preservation medium', BMJ Open Ophthalmology, vol. 4, no. 1, e000246. https://doi.org/10.1136/bmjophth-2018-000246
dc.identifier.doi10.1136/bmjophth-2018-000246
dc.identifier.issn2397-3269
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/17012
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85066735229&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofBMJ Open Ophthalmology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subject16S
dc.subject18S
dc.subjectbacteria
dc.subjectcornea
dc.subjecteye bank
dc.subjectfungus
dc.subjectmedia
dc.subjectmicrobiology
dc.subjectNGS
dc.subjectpreservation
dc.subjectstorage
dc.subject3.2 Clinical medicine
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectOphthalmology
dc.titleNext-generation sequencing for the detection of microorganisms present in human donor corneal preservation mediumen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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