A unique borrelial protein facilitates microbial immune evasion

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dc.contributor.authorFoor, Shelby D
dc.contributor.authorBrangulis, Kalvis
dc.contributor.authorShakya, Anil K
dc.contributor.authorRana, Vipin S
dc.contributor.authorBista, Sandhya
dc.contributor.authorKitsou, Chrysoula
dc.contributor.authorRonzetti, Michael
dc.contributor.authorAlreja, Adit B
dc.contributor.authorLinden, Sara B
dc.contributor.authorAltieri, Amanda S
dc.contributor.authorBaljinnyam, Bolormaa
dc.contributor.authorAkopjana, Inara
dc.contributor.authorNelson, Daniel C
dc.contributor.authorSimeonov, Anton
dc.contributor.authorHerzberg, Osnat
dc.contributor.authorCaimano, Melissa J
dc.contributor.authorPal, Utpal
dc.contributor.institutionDepartment of Human Physiology and Biochemistry
dc.date.accessioned2023-11-23T13:45:04Z
dc.date.available2023-11-23T13:45:04Z
dc.date.issued2023-10-31
dc.descriptionPublisher Copyright: © 2023 American Society for Microbiology. All rights reserved.
dc.description.abstractBorrelia burgdorferi, the pathogen of Lyme disease, encodes many conserved proteins of unknown structure or function, including ones that serve essential roles in microbial infectivity. One such protein is BB0238, which folds into a two-domain protein, as we have determined by X-ray crystallography and AlphaFold analysis. The N-terminal domain begins with a helix-turn-helix motif (HTH), previously referred to as a tetratricopeptide repeat (TPR) motif, known to mediate protein-protein interactions. The fold of the C-terminal domain has been seen in proteins with a range of unrelated activities and thus does not infer function. In addition to its previously known binding partner BB0323, another essential borrelial virulence determinant, we show that BB0238 also binds a second protein, BB0108, a borrelial ortholog of the chaperone protein SurA and the peptidyl-prolyl cis/ trans isomerase protein PrsA. An in vitro enzymatic assay confirmed the catalytic activity. We also determined the crystal structure of the catalytic domain of BB0108, which revealed the parvulin-type organization of the key catalytic residues. We show that BB0238 influences the proteolytic processing of BB0323, although the TPR/HTH motif is not involved in the process. Instead, we show that the motif stabilizes BB0238 in the host environment and facilitates tick-to-mouse pathogen transmission by aiding spirochete evasion of early host cellular immunity. Taken together, these studies highlight the biological significance of BB0238 and its interactions with multiple B. burgdorferi proteins essential for microbial infection. IMPORTANCE Lyme disease is a major tick-borne infection caused by a bacterial pathogen called Borrelia burgdorferi, which is transmitted by ticks and affects hundreds of thousands of people every year. These bacterial pathogens are distinct from other genera of microbes because of their distinct features and ability to transmit a multi-system infection to a range of vertebrates, including humans. Progress in understanding the infection biology of Lyme disease, and thus advancements towards its prevention, are hindered by an incomplete understanding of the microbiology of B. burgdorferi, partly due to the occurrence of many unique borrelial proteins that are structurally unrelated to proteins of known functions yet are indispensable for pathogen survival. We herein report the use of diverse technologies to examine the structure and function of a unique B. burgdorferi protein, annotated as BB0238-an essential virulence determinant. We show that the protein is structurally organized into two distinct domains, is involved in multiplex protein-protein interactions, and facilitates tick-to-mouse pathogen transmission by aiding microbial evasion of early host cellular immunity. We believe that our findings will further enrich our understanding of the microbiology of B. burgdorferi, potentially impacting the future development of novel prevention strategies against a widespread tick-transmitted infection.en
dc.description.statusPeer reviewed
dc.format.extent24
dc.format.extent3573466
dc.identifier.citationFoor, S D, Brangulis, K, Shakya, A K, Rana, V S, Bista, S, Kitsou, C, Ronzetti, M, Alreja, A B, Linden, S B, Altieri, A S, Baljinnyam, B, Akopjana, I, Nelson, D C, Simeonov, A, Herzberg, O, Caimano, M J & Pal, U 2023, 'A unique borrelial protein facilitates microbial immune evasion', mBio, vol. 14, no. 5, e0213523. https://doi.org/10.1128/mbio.02135-23
dc.identifier.doi10.1128/mbio.02135-23
dc.identifier.issn2161-2129
dc.identifier.otherunpaywall: 10.1128/mbio.02135-23
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/14982
dc.identifier.urlhttps://www-webofscience-com.db.rsu.lv/wos/alldb/full-record/WOS:001084696500001
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85176736065&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofmBio
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBorrelia burgdorferi
dc.subjecttick-borne pathogens
dc.subjectimmune evasion
dc.subject1.6 Biological sciences
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.titleA unique borrelial protein facilitates microbial immune evasionen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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