Cancer microcell initiation and determination

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dc.contributor.authorSimsone, Zane
dc.contributor.authorFreivalds, Tālivaldis
dc.contributor.authorBēma, Dina
dc.contributor.authorMiķelsone, Indra
dc.contributor.authorPatetko, Liene
dc.contributor.authorBērziņš, Juris
dc.contributor.authorHarju, Līga
dc.contributor.authorBuiķis, Indulis
dc.contributor.institutionDepartment of Human Physiology and Biochemistry
dc.date.accessioned2021-12-22T14:30:01Z
dc.date.available2021-12-22T14:30:01Z
dc.date.issued2021-12
dc.descriptionFunding Information: This project was supported by the University of Latvia Donor SIA “Mikrotīkls.” The University of Latvia Foundation administered this donation. Funding Information: This research was accomplished with the support of the Institute of Cardiology and Regenerative Medicine, University of Latvia. The cell culture cultivation was carried out in cooperation with the Latvian Biomedical Research and Study Centre. The confocal microscopy was carried out in collaboration with Carl Zeiss Microscopy Democenter Oberkochen, Germany. Publisher Copyright: © 2021, The Author(s).
dc.description.abstractBackground: Cancer remains one of the leading causes of death worldwide, despite the possibilities to detect early onset of the most common cancer types. The search for the optimal therapy is complicated by the cancer diversity within tumors and the unsynchronized development of cancerous cells. Therefore, it is necessary to characterize cancer cell populations after treatment has been applied, because cancer recurrence is not rare. In our research, we concentrated on small cancer cell subpopulation (microcells) that has a potential to be cancer resistance source. Previously made experiments has shown that these cells in small numbers form in specific circumstances after anticancer treatment. Methods: In experiments described in this research, the anticancer agents’ paclitaxel and doxorubicin were used to stimulate the induction of microcells in fibroblast, cervix adenocarcinoma, and melanoma cell lines. Mainly for the formation of microcells in melanoma cells. The drug-stimulated cells were then characterized in terms of their formation efficiency, morphology, and metabolic activity. Results: We observed the development of cancer microcells and green fluorescent protein (GFP) transfection efficiency after stress. In the time-lapse experiment, we observed microcell formation through a renewal process and GFP expression in the microcells. Additionally, the microcells were viable after anticancer treatment, as indicated by the nicotinamide adenine dinucleotide hydrogen phosphate (NADPH) enzyme activity assay results. Taken together, these findings indicate that cancer microcells are viable and capable of resisting the stress induced by anticancer drugs, and these cells are prone to chemical substance uptake from the environment. Conclusion: Microcells are not only common to a specific cancer type, but can be found in any tumor type. This study could help to understand cancer emergence and recurrence. The appearance of microcells in the studied cancer cell population could be an indicator of the individual anticancer therapy effectiveness and patient survival.en
dc.description.statusPeer reviewed
dc.format.extent15
dc.format.extent10946199
dc.identifier.citationSimsone, Z, Freivalds, T, Bēma, D, Miķelsone, I, Patetko, L, Bērziņš, J, Harju, L & Buiķis, I 2021, 'Cancer microcell initiation and determination', BMC Cancer, vol. 21, no. 1, 1087. https://doi.org/10.1186/s12885-021-08813-5
dc.identifier.doi10.1186/s12885-021-08813-5
dc.identifier.issn1471-2407
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/7120
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85116824378&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofBMC Cancer
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectcancer
dc.subjectcancer resistance
dc.subjectCell viability
dc.subjectDoxorubicin
dc.subjectMicrocell
dc.subjectNADPH
dc.subjectPaclitaxel
dc.subjectSK-MEL-28
dc.subject3.1 Basic medicine
dc.subject3.2 Clinical medicine
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectOncology
dc.subjectGenetics
dc.subjectCancer Research
dc.subjectSDG 3 - Good Health and Well-being
dc.titleCancer microcell initiation and determinationen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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