Possible chromosomal and germline integration of human herpesvirus 7

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dc.contributor.authorPrusty, Bhupesh K.
dc.contributor.authorGulve, Nitish
dc.contributor.authorRasa, Santa
dc.contributor.authorMurovska, Modra
dc.contributor.authorHernandez, Pilar Collado
dc.contributor.authorAblashi, Dharam V.
dc.contributor.institutionInstitute of Microbiology and Virology
dc.date.accessioned2021-04-20T11:00:01Z
dc.date.available2021-04-20T11:00:01Z
dc.date.issued2017-02
dc.descriptionPublisher Copyright: © 2017 The Authors. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.
dc.description.abstractHuman herpesvirus 7 (HHV-7) is a betaherpesvirus, and is phylogenetically related to both HHV-6A and HHV-6B. The presence of telomeric repeat sequences at both ends of its genome should make it equally likely to integrate into the human telomere as HHV-6. However, numerous studies have failed to detect germline integration of HHV-7, suggesting an important difference between the HHV-6A/-6B and HHV-7 genomes. In search of possible germline integrated HHV-7, we developed a sensitive and quantitative real-time PCR assay and discovered that primers designed against some parts of the HHV-7 genome can frequently miss HHV-7 positive clinical samples even though they work efficiently in cell-culture-derived HHV-7 positive materials. Using a primer pair against the U90 ORF of HHV-7, we identified a possible case of germline integration of HHV-7 with one copy of viral genome per cell in both peripheral blood cells and hair follicles. Chromosomal integration of HHV-7 in these individuals was confirmed by fluorescence in situ hybridization analysis. Germline integration of HHV-7 was further confirmed by detection of ~2.6 copies of HHV-7 in the hair follicles of one of the parents. Our results shed light on the complex nature of the HHV-7 genome in human-derived materials in comparison to cell-culture-derived materials and show the need for stringent criteria in the selection of primers for epidemiological HHV-7 studies.en
dc.description.statusPeer reviewed
dc.format.extent9
dc.format.extent3100312
dc.identifier.citationPrusty, B K, Gulve, N, Rasa, S, Murovska, M, Hernandez, P C & Ablashi, D V 2017, 'Possible chromosomal and germline integration of human herpesvirus 7', Journal of General Virology, vol. 98, no. 2, pp. 266-274. https://doi.org/10.1099/jgv.0.000692
dc.identifier.doi10.1099/jgv.0.000692
dc.identifier.issn0022-1317
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/3861
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85015703561&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofJournal of General Virology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectChromosomal integration
dc.subjectciHHV-6
dc.subjectHHV-6
dc.subjectHHV-7
dc.subject1.6 Biological sciences
dc.subject3.3 Health sciences
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectVirology
dc.titlePossible chromosomal and germline integration of human herpesvirus 7en
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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