In vitro impact preliminary assessment of airborne particulate from metalworking and woodworking industries

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dc.contributor.authorPavlovska, Ilona
dc.contributor.authorRamata-Stunda, Anna
dc.contributor.authorMartinsone, Žanna
dc.contributor.authorBoroduskis, Martins
dc.contributor.authorPatetko, Liene
dc.contributor.authorMārtiņsone, Inese
dc.contributor.authorSeile, Anita
dc.contributor.authorVanadziņš, Ivars
dc.contributor.institutionInstitute of Occupational Safety and Environmental Health
dc.date.accessioned2021-10-15T09:05:01Z
dc.date.available2021-10-15T09:05:01Z
dc.date.issued2021-10-12
dc.descriptionFunding Information: This work was supported by ESF project “Development of up-to-date diagnostic and research methods for the risks caused by nanoparticles and ergonomic factors at workplaces” Agreement No. 2013/0050/1DP/1.1.1.2.0/13/ APIA/VIAA/025. Publisher Copyright: © 2021, The Author(s).
dc.description.abstractInhalation is the main route of exposure to airborne pollutants. To evaluate the safety and assess the risks of occupational hazards different testing approaches are used. 3D airway epithelial tissues allow to mimic exposure conditions in vitro, generates human-relevant toxicology data, allows to elucidate the mode of action of pollutants. Gillian3500 pumps were used to collect the airborne particulate from woodworking and metalworking environments. EpiAirway tissues were used to model half working day (4 h), full working day (8 h), and 3 working day exposures to occupational pollutants. Tissue viability was assessed using an MTT assay. For preliminary assessment, RT-qPCR analyses were performed to analyze the expression of gelsolin, caspase-3, and IL-6. Tissue morphology was assessed by hematoxylin/eosin staining. An effect on the proliferation of lung epithelial cell line A549 was assessed. Acute exposure to workspace pollutants slightly affected tissue viability and did not change the morphology. No inhibiting effect was observed on the proliferation of A549 cells. Preliminary analysis showed that both types of particles suppressed the expression of gelsolin, with the effect of metalworking samples being more pronounced. A slight reduction in caspase-3 expression was observed. Particles from metalworking suppressed IL-6 expression.en
dc.description.statusPeer reviewed
dc.format.extent11
dc.format.extent2000800
dc.identifier.citationPavlovska, I, Ramata-Stunda, A, Martinsone, Ž, Boroduskis, M, Patetko, L, Mārtiņsone, I, Seile, A & Vanadziņš, I 2021, 'In vitro impact preliminary assessment of airborne particulate from metalworking and woodworking industries', Scientific Reports, vol. 11, no. 1, 20181, pp. 20181. https://doi.org/10.1038/s41598-021-99815-7
dc.identifier.doi10.1038/s41598-021-99815-7
dc.identifier.issn2045-2322
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/6653
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85116924996&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofScientific Reports
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subject2.4 Chemical engineering
dc.subject1.5 Earth and related Environmental sciences
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.titleIn vitro impact preliminary assessment of airborne particulate from metalworking and woodworking industriesen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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