Morphological and Immunocytochemical Characterization of Paclitaxel-Induced Microcells in Sk-Mel-28 Melanoma Cells

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dc.contributor.authorSimsone, Zane
dc.contributor.authorFreivalds, Talivaldis
dc.contributor.authorHarju, Līga
dc.contributor.authorMiķelsone, Indra
dc.contributor.authorBlāķe, Ilze
dc.contributor.authorBērziņš, Juris
dc.contributor.authorBuiķis, Indulis
dc.contributor.institutionDepartment of Human Physiology and Biochemistry
dc.date.accessioned2024-07-19T09:55:01Z
dc.date.available2024-07-19T09:55:01Z
dc.date.issued2024-07
dc.descriptionPublisher Copyright: © 2024 by the authors.
dc.description.abstractBiomarkers, including proteins, nucleic acids, antibodies, and peptides, are essential for identifying diseases such as cancer and differentiating between healthy and abnormal cells in patients. To date, studies have shown that cancer stem cells have DNA repair mechanisms that deter the effects of medicinal treatment. Experiments with cell cultures and chemotherapy treatments of these cultures have revealed the presence of small cells, with a small amount of cytoplasm that can be intensively stained with azure eosin, called microcells. Microcells develop during sporosis from a damaged tumor macrocell. After anticancer therapy in tumor cells, a defective macrocell may produce one or more microcells. This study aims to characterize microcell morphology in melanoma cell lines. In this investigation, we characterized the population of cancer cell microcells after applying paclitaxel treatment to a Sk-Mel-28 melanoma cell line using immunocytochemical cell marker detection and fluorescent microscopy. Paclitaxel-treated cancer cells show stronger expression of stem-associated ALDH2, SOX2, and Nanog markers than untreated cells. The proliferation of nuclear antigens in cells and the synthesis of RNA in microcells indicate cell self-defense, promoting resistance to applied therapy. These findings improve our understanding of microcell behavior in melanoma, potentially informing future strategies to counteract drug resistance in cancer treatment.en
dc.description.statusPeer reviewed
dc.format.extent19
dc.format.extent28871521
dc.identifier.citationSimsone, Z, Freivalds, T, Harju, L, Miķelsone, I, Blāķe, I, Bērziņš, J & Buiķis, I 2024, 'Morphological and Immunocytochemical Characterization of Paclitaxel-Induced Microcells in Sk-Mel-28 Melanoma Cells', Biomedicines, vol. 12, no. 7, 1576. https://doi.org/10.3390/biomedicines12071576
dc.identifier.doi10.3390/biomedicines12071576
dc.identifier.issn2227-9059
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/16470
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85199668725&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofBiomedicines
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectcancer biology
dc.subjectearly detection
dc.subjectnew paradigms
dc.subjectmicrocells
dc.subjectmacrocells
dc.subjectsporosis
dc.subjectPaclitaxel
dc.subjectSOX2
dc.subjectPCNA
dc.subjectcaspases
dc.subjectNanog
dc.subject1.6 Biological sciences
dc.subject3.1 Basic medicine
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectSDG 3 - Good Health and Well-being
dc.titleMorphological and Immunocytochemical Characterization of Paclitaxel-Induced Microcells in Sk-Mel-28 Melanoma Cellsen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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