Expression of ORAI1 and STIM1 genes in blood of patients with pulmonary tuberculosis

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dc.contributor.authorKolesova, Oksana
dc.contributor.authorKramica, Ksenija
dc.contributor.authorKolesovs, Aleksandrs
dc.contributor.authorEglite, Jelena
dc.contributor.institutionRīga Stradiņš University
dc.date.accessioned2022-08-16T21:02:04Z
dc.date.available2022-08-16T21:02:04Z
dc.date.issued2021
dc.descriptionFunding Information: This study was supported by Rl-ga Stradin¸š University, Latvia, Grant No. 21-1/1/2019-4. Publisher Copyright: © 2021 Termedia Publishing House Ltd.. All rights reserved.
dc.description.abstractThis study aimed to detect the expression level of ORAI1 and STIM1 genes in blood of patients with bilateral pulmonary tuberculosis (TB) in comparison with the control group. Both genes encode proteins providing store-operated Ca2+ entry (SOCE) into the cells, including immune cells, to activate transcriptional factors for producing cytokines and inflammation-restricting proteins. The study included 45 patients with confirmed TB, aged 20 to 86, and 35 volunteers, aged from 21 to 73, without active TB infection. The expression of ORAI1 and STIM1 genes in blood was performed by real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the referent gene. Inflammation was assessed by levels of interferon γ (IFN-γ) and interleukin 18 (IL-18) in serum (ELISA method). The results showed lower expression of ORAI1 in blood and higher levels of IFN-γ and IL-18 in serum of TB patients than that of the control group and no differences in expression of the STIM1 gene. It indicates some impairment in the SOCE mechanism of immune cells, which is associated with TB.en
dc.description.statusPeer reviewed
dc.format.extent8
dc.format.extent305715
dc.identifier.citationKolesova, O, Kramica, K, Kolesovs, A & Eglite, J 2021, 'Expression of ORAI1 and STIM1 genes in blood of patients with pulmonary tuberculosis', Central European Journal of Immunology, vol. 46, no. 2, pp. 275-282. https://doi.org/10.5114/CEJI.2021.106998
dc.identifier.doi10.5114/CEJI.2021.106998
dc.identifier.issn1426-3912
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/9365
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85116196733&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofCentral European Journal of Immunology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCytokines
dc.subjectMRNA ORAI1
dc.subjectMRNA STIM1
dc.subjectSOCE
dc.subjectTuberculosis
dc.subject1.6 Biological sciences
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectImmunology and Allergy
dc.subjectImmunology
dc.subjectSDG 3 - Good Health and Well-being
dc.titleExpression of ORAI1 and STIM1 genes in blood of patients with pulmonary tuberculosisen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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