Vaccinium myrtillus un Vaccinium corymbosum ekstraktu fitoķīmiska izpēte un antibakteriālās aktivitātes noteikšana.
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Date
2023
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Rīgas Stradiņa universitāte
Rīga Stradiņš University
Rīga Stradiņš University
Abstract
Ārstniecības augi un no tiem gatavoti preparāti jau gadsimtiem ilgi tiek izmantoti gan dažādu veselības problēmu ārstēšanai, gan to pofilaksei. Ārstniecības augu pielietošana uz pierādījumiem balstītā medicīnā balstās uz to fitoķīmisko sastāvu. Vaccinium ģints augi ir vieni no bagātākajiem dabīgajiem polifenolu avotiem, tādēļ tiem potenciāli var piemist antibakteriālā aktivitāte. Lai gan Vaccinium ģints auga, Vaccinium oxycoccus (dzērvene), fitoķīmiskais profils un antibakteriālā aktivitāte ir literatūrā plaši aprakstīta, šobrīd pieejamie dati par 2 citām šīs ģints sugām - Vaccinium myrtillus (mellene) un Vaccinium corymbosum (vairogu zilene) nav pietiekami un viennozīmīgi. Šī pētījuma mērķis ir izpētīt Latvijas izcelsmes melleņu un vairogu zileņu ekstraktu ķīmisku sastāvu un noteikt to antibakteriālo aktivitāti.
Kopumā tika ievākti 13 ogu paraugi un, izmantojot macerācijas metodi, tika pagatavoti ekstrakti, katram paraugam pagatavojot 3 paralēlos ekstraktus. Fitoķīmiska sastāva izpētei tika izmantotas Furjē transformācijas infrasarkanās spektroskopijas, UV/VIS spektroskopijas, augstas efektivitātes šķidruma hromatogrāfijas un plānslāņa hromatogrāfijas metodes. Antibakteriālā aktivitāte tika noteikta pret diviem baktēriju celmiem – S. aureus (Gr+) un E. coli (Gr-).
Iegūtie rezultāti liecina, ka kopējais polifenolu saturs melleņu ģints augos variē no 20,06 mg galusskābes ekvivalenta/g liofilizēta ekstrakta (Vaccinium corymbosum) līdz 57,11 mg galluskābes ekvivalenta/g liofilizēta ekstrakta (Vaccinium myrtillus). Abu sugu dominējošā fenolskābe ir hlorogēnskābe, bet tika novērotas būtiskas atšķirības hlorogēnskābes koncentrācijās starp šīm sugām. Ekstrakti uzrādīja no devas atkarīgus augšanu inhibējošus efektus pret S.aureus un E.coli baktērijām.
Abu petīto sugu augļi satur ievērojamu daudzumu polifenolu un to ekstraktiem piemīt antibakteriālā aktivitāte gan pret S.aureus, gan pret E.coli baktēriju celmiem. Nepieciešami tālāki pētījumi par šo sugu augļu potenciālu pielietojumu uz pierādījumiem balstītā medicīnā.
Medicinal plants and their preparations have been used for centuries both as a prophylactic and therapeutic tool against various health problems. The use of medicinal plants in evidence-based medicine is based on their phytochemical composition. Vaccinium genus plants are one of the richest natural sources of polyphenols, therefore potentially having antimicrobial activity. Although the antimicrobial activity and phytochemical profile of the Vaccinium genus plant Vaccinium oxycoccus (cranberry) have been widely discussed in the literature, currently the data available on 2 other species of this genus - Vaccinium myrtillus (bilberry) and Vaccinium corymbosum (highbush blueberry) isn’t sufficient and unambiguous. This research aimed to investigate the chemical composition of bilberry and highbush blueberry extracts of Latvian origin and to determine their antimicrobial activity. A total of 13 berry samples were collected and extraction using maceration was carried out in triplicate for each sample. Fourier transform infrared spectroscopy, UV/VIS spectroscopy, high-performance liquid chromatography, and thin layer chromatography methods were used to determine the phytochemical composition. Antimicrobial activity was determined against two bacterial strains - S. aureus (Gr+) and E. coli (Gr-). Data shows that the total polyphenol content in blueberry genus plants varies from 20,06 mg gallic acid equivalent/g lyophilized extract (Vaccinium corymbosum) to 57,11 mg gallic acid equivalent/g lyophilized extract (Vaccinium myrtillus). The dominant phenolic acid in both species were chlorogenic acid, but significant differences in concentrations were observed between these species. Dose-dependant growth-inhibitory effects of extracts against S.aureus and E.coli were observed. Fruits of studied species of the blueberry genus contain a high amount of polyphenols and their extracts show antibacterial activity against both S.aureus and E.coli bacterial strains. Further research into the potential applications of the fruits of these species in evidence-based medicine is needed.
Medicinal plants and their preparations have been used for centuries both as a prophylactic and therapeutic tool against various health problems. The use of medicinal plants in evidence-based medicine is based on their phytochemical composition. Vaccinium genus plants are one of the richest natural sources of polyphenols, therefore potentially having antimicrobial activity. Although the antimicrobial activity and phytochemical profile of the Vaccinium genus plant Vaccinium oxycoccus (cranberry) have been widely discussed in the literature, currently the data available on 2 other species of this genus - Vaccinium myrtillus (bilberry) and Vaccinium corymbosum (highbush blueberry) isn’t sufficient and unambiguous. This research aimed to investigate the chemical composition of bilberry and highbush blueberry extracts of Latvian origin and to determine their antimicrobial activity. A total of 13 berry samples were collected and extraction using maceration was carried out in triplicate for each sample. Fourier transform infrared spectroscopy, UV/VIS spectroscopy, high-performance liquid chromatography, and thin layer chromatography methods were used to determine the phytochemical composition. Antimicrobial activity was determined against two bacterial strains - S. aureus (Gr+) and E. coli (Gr-). Data shows that the total polyphenol content in blueberry genus plants varies from 20,06 mg gallic acid equivalent/g lyophilized extract (Vaccinium corymbosum) to 57,11 mg gallic acid equivalent/g lyophilized extract (Vaccinium myrtillus). The dominant phenolic acid in both species were chlorogenic acid, but significant differences in concentrations were observed between these species. Dose-dependant growth-inhibitory effects of extracts against S.aureus and E.coli were observed. Fruits of studied species of the blueberry genus contain a high amount of polyphenols and their extracts show antibacterial activity against both S.aureus and E.coli bacterial strains. Further research into the potential applications of the fruits of these species in evidence-based medicine is needed.
Description
Farmācija
Pharmacy
Veselības aprūpe
Health Care
Pharmacy
Veselības aprūpe
Health Care
Keywords
Vaccinium myrtillus, Vaccinium corymbosum, antibakteriālā aktivitāte, polifenoli, fenolskābes, flavonoīdi, Vaccinium myrtillus, Vaccinium corymbosum, antibacterial activity, polyphenols, phenolic acids, flavonoids