Evaluation of the Possibility to Detect Circulating Tumor DNA From Pituitary Adenoma

Simple item page
dc.contributor.authorMegnis, Kaspars
dc.contributor.authorPeculis, Raitis
dc.contributor.authorRovite, Vita
dc.contributor.authorLaksa, Pola
dc.contributor.authorNiedra, Helvijs
dc.contributor.authorBalcere, Inga
dc.contributor.authorCaune, Olivija
dc.contributor.authorBreiksa, Austra
dc.contributor.authorNazarovs, Jurijs
dc.contributor.authorStukens, Janis
dc.contributor.authorKonrade, Ilze
dc.contributor.authorPirags, Valdis
dc.contributor.authorKlovins, Janis
dc.contributor.institutionDepartment of Internal Diseases
dc.date.accessioned2021-04-21T07:00:01Z
dc.date.available2021-04-21T07:00:01Z
dc.date.issued2019-09-18
dc.descriptionFunding Information: The authors acknowledge the Latvian Biomedical Research and Study Center and the Genome Database of the Latvian Population for providing infrastructure, biological material, and data. Funding. This research was supported by the European Regional Development Fund (ERDF), Measure 1.1.1.1 Industry-Driven Research project Molecular markers of pituitary tumor development, progression and therapy response (Project No. 1.1.1.1/16/A/066, 2017), and Roche Academy project Immunohistochemical analysis of pituitary adenoma tissue. Publisher Copyright: © Copyright © 2019 Megnis, Peculis, Rovite, Laksa, Niedra, Balcere, Caune, Breiksa, Nazarovs, Stukens, Konrade, Pirags and Klovins.
dc.description.abstractObjective: Circulating free DNA (cfDNA) in general and circulating tumor DNA (ctDNA) in particular is becoming an increasingly used form of liquid biopsy biomarkers. In this study, we are investigating the ability to detect ctDNA from the plasma of pituitary adenoma (PA) patients. Design: Tumor tissue samples were obtained from planed PA resections, before which blood plasma samples were taken. Somatic variants found in PA tissue samples were evaluated in related cfDNA, isolated from plasma samples. Methods: Sanger sequencing, as well as previously obtained whole-exome sequencing data, were used to evaluate somatic variants composition in tumor tissue samples. cfDNA was isolated from the same PA patients and competitive allele-specific TaqMan PCR and amplicon-based next-generation sequencing (NGS) approach were used for targeted detection of variants found in corresponding tumor tissue samples. Results: Using NGS-based analysis, we detected five out of 17 somatic variants in 40 to 60% of total reads, three variants in 0.50–5.00% of total read count, including GNAS c.601C>T, which was detected using ultra-deep NGS (1.78 million X) in 0.77% of amplicons reads. Nine variants were not detected. We also detected We were not able to detect variant found in PA tissue in cfDNA using cast-PCR, indicating that the portion of variant-containing ctDNA in total isolated cfDNA is too small to be detected with this method. Conclusions: For the first time, we demonstrate the possibility to detect somatic variants of PA in cfDNA isolated from patients' blood plasma. Whether the source of variant detected in cfDNA is PA should be further tested.en
dc.description.statusPeer reviewed
dc.format.extent362957
dc.identifier.citationMegnis, K, Peculis, R, Rovite, V, Laksa, P, Niedra, H, Balcere, I, Caune, O, Breiksa, A, Nazarovs, J, Stukens, J, Konrade, I, Pirags, V & Klovins, J 2019, 'Evaluation of the Possibility to Detect Circulating Tumor DNA From Pituitary Adenoma', Frontiers in Endocrinology, vol. 10, 615. https://doi.org/10.3389/fendo.2019.00615
dc.identifier.doi10.3389/fendo.2019.00615
dc.identifier.issn1664-2392
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/3875
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85072882132&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofFrontiers in Endocrinology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectcirculating tumor DNA
dc.subjectcompetitive allele-specific TaqMan
dc.subjectGNAS
dc.subjectnext-generation sequencing
dc.subjectpituitary adenoma
dc.subject3.1 Basic medicine
dc.subject3.2 Clinical medicine
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectEndocrinology, Diabetes and Metabolism
dc.subjectSDG 3 - Good Health and Well-being
dc.titleEvaluation of the Possibility to Detect Circulating Tumor DNA From Pituitary Adenomaen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Evaluation_of_the_Possibility_to_Detect_Circulating_Tumor_DNA.pdf
Size:
354.45 KB
Format:
Adobe Portable Document Format
Download

Collections

Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure