Characterization of cytokines and proliferation marker Ki67 in cleft affected lip tissue

Simple item page
dc.contributor.authorPilmane, Mara
dc.contributor.authorSidhoma, Elga
dc.contributor.authorAkota, Ilze
dc.contributor.authorKazoka, Dzintra
dc.contributor.institutionInstitute of Anatomy and Anthropology
dc.contributor.institutionRīga Stradiņš University
dc.date.accessioned2021-04-12T08:35:01Z
dc.date.available2021-04-12T08:35:01Z
dc.date.issued2019-09
dc.descriptionFunding Information: Funding: This research was funded by Riga Stradins University, Nr.5-1/106/2019 from 12.04.2019 “The elaboration of diagnostic prognostic factor algorithm of facial tissue complex regeneration for cleft patients in longitudinal aspect”. Publisher Copyright: © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
dc.description.abstractBackground and objectives: Cleft lip palate takes the second place among all anomalies. The complex appearance of cytokines and proliferation markers has still not been clarified despite their possible crucial role in cleft tissue. Therefore, the aim of work was the detection of appearance of pro- and anti-inflammatory cytokines and proliferation marker Ki67, and their inter-correlations in cleft affected lip (CAL). Materials and Methods: The lip material was obtained from 16 children aged before primary dentition during plastic surgery. Control was obtained from 7 non-CAL oral tissue. Tissues were stained for IL-1, IL-4, IL-6, IL-8, IL-10 and Ki67 immunohistochemically. Nonparametric statistic, Mann-Whitney and Spearman’s coefficient were used. Results: All cytokines positive cells were observed more into the epithelium. Statistically significant difference was seen between epithelial IL-1, IL-10, IL-8 and Ki67 positive cells and IL-10-, IL-4-containing connective tissue cells in comparison to the control. Strong positive correlation was detected in CAL epithelium between IL-10 and IL-8, IL-10 and IL-4, IL-10 and IL-1, IL-1 and IL-8, IL-1 and IL-4, IL-4 and IL-8, IL-8 and Ki67, IL-10 and Ki67, but moderate-in connective tissue between IL-1 and IL-10, IL-1 and IL-4. Conclusion: The CAL epithelium is the main source for the interleukins. Rich similar expression of IL-1 and IL-10 suggests the balance between pro-and anti-inflammatory tissue response on basis of dysregulated tissue homeostasis (increase of IL-8). The correlations between the different ILs -1, -4, -8, -10 in CAL epithelium seem to indicate the self-protection compensatory mechanism for intensification of local inflammatory-immune response without involvement of IL-6. The correlations between Ki67 and cytokines indicate the involvement of IL-8 and IL-10 in stimulation of cellular proliferation. IL-4 and IL-10 expression from CAL connective tissue simultaneously to IL-1, IL-4 and IL-10 inter-correlations there suggests the intensification of local immune response regulated probably by main pro-inflammatory cytokine-IL-1.en
dc.description.statusPeer reviewed
dc.format.extent9594299
dc.identifier.citationPilmane, M, Sidhoma, E, Akota, I & Kazoka, D 2019, 'Characterization of cytokines and proliferation marker Ki67 in cleft affected lip tissue', Medicina (Lithuania), vol. 55, no. 9, 518. https://doi.org/10.3390/medicina55090518
dc.identifier.doi10.3390/medicina55090518
dc.identifier.issn1010-660X
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/3738
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85071467665&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofMedicina (Lithuania)
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectChildren
dc.subjectCleft
dc.subjectCytokines
dc.subjectLip
dc.subjectProliferation marker
dc.subject3.1 Basic medicine
dc.subject3.2 Clinical medicine
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectGeneral Medicine
dc.titleCharacterization of cytokines and proliferation marker Ki67 in cleft affected lip tissueen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Characterization_of_cytokines.pdf
Size:
9.15 MB
Format:
Adobe Portable Document Format
Download

Collections

Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure