Development of single-tube nested real-time PCR assays with long internally quenched probes for detection of norovirus genogroup II

Simple item page
dc.contributor.authorXia, Hongyan
dc.contributor.authorGravelsina, Sabine
dc.contributor.authorÖhrmalm, Christina
dc.contributor.authorOttoson, Jakob
dc.contributor.authorBlomberg, Jonas
dc.contributor.institutionInstitute of Microbiology and Virology
dc.date.accessioned2021-06-28T06:35:01Z
dc.date.available2021-06-28T06:35:01Z
dc.date.issued2016-01
dc.descriptionPublisher Copyright: © 2016, Eaton Publishing Company. All rights reserved.
dc.description.abstractThe high sequence variation of RNA viruses necessitates use of degenerate primers and probes or multiple primers and probes in molecular diagnostic assays. We showed previously that PCR amplification in two rounds, first with long target-specific primers and then with short generic primers, followed by detection using long probes, can tolerate sequence variation. Here we demonstrate that long primers and probes of up to 56 nucleotides can also be applied in real-time PCR for the detection of norovirus genogroup II with improved sensitivity. Probe design (method of incorporating quenchers, use of Zen internal quencher or traditional quenchers) greatly affects the sensitivity of the real-time PCR assays.en
dc.description.statusPeer reviewed
dc.format.extent7
dc.format.extent2650619
dc.identifier.citationXia, H, Gravelsina, S, Öhrmalm, C, Ottoson, J & Blomberg, J 2016, 'Development of single-tube nested real-time PCR assays with long internally quenched probes for detection of norovirus genogroup II', BioTechniques, vol. 60, no. 1, pp. 28-34. https://doi.org/10.2144/000114370
dc.identifier.doi10.2144/000114370
dc.identifier.issn0736-6205
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/5603
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=84954307287&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofBioTechniques
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectNorovirus
dc.subjectReal-time PCR
dc.subjectVariation-tolerant capture multiplex assay
dc.subject1.6 Biological sciences
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectBiotechnology
dc.subjectGeneral Biochemistry,Genetics and Molecular Biology
dc.titleDevelopment of single-tube nested real-time PCR assays with long internally quenched probes for detection of norovirus genogroup IIen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Development_of_single_tube_nested_real_time_PCR_assays_with_long_internally.pdf
Size:
2.53 MB
Format:
Adobe Portable Document Format
Download

Collections

Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure