Plasma and urinary extracellular vesicles as a source of RNA biomarkers for prostate cancer in liquid biopsies

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dc.contributor.authorBajo-Santos, Cristina
dc.contributor.authorBrokāne, Agnese
dc.contributor.authorZayakin, Pawel
dc.contributor.authorEndzeliņš, Edgars
dc.contributor.authorSoboļevska, Kristīne
dc.contributor.authorBelovs, Alberts
dc.contributor.authorJansons, Juris
dc.contributor.authorSperga, Māris
dc.contributor.authorLlorente, Alicia
dc.contributor.authorRadoviča-Spalviņa, Ilze
dc.contributor.authorLietuvietis, Vilnis
dc.contributor.authorLinē, Aija
dc.contributor.institutionRīga Stradiņš University
dc.date.accessioned2024-02-02T13:35:01Z
dc.date.available2024-02-02T13:35:01Z
dc.date.issued2023-02-03
dc.descriptionPublisher Copyright: Copyright © 2023 Bajo-Santos, Brokāne, Zayakin, Endzeliņš, Soboļevska, Belovs, Jansons, Sperga, Llorente, Radoviča-Spalviņa, Lietuvietis and Linē.
dc.description.abstractIntroduction: Extracellular vesicles (EVs) have emerged as a very attractive source of cancer- derived RNA biomarkers for the early detection, prognosis and monitoring of various cancers, including prostate cancer (PC). However, biofluids contain a mixture of EVs released from a variety of tissues and the fraction of total EVs that are derived from PC tissue is not known. Moreover, the optimal biofluid—plasma or urine—that is more suitable for the detection of EV- enclosed RNA biomarkers is not yet clear. Methodology: In the current study, we performed RNA sequencing analysis of plasma and urinary EVs collected before and after radical prostatectomy, and matched tumor and normal prostate tissues of 10 patients with prostate cancer. Results and Discussion: The most abundant RNA biotypes in EVs were miRNA, piRNA, tRNA, lncRNA, rRNA and mRNA. To identify putative cancer-derived RNA biomarkers, we searched for RNAs that were overexpressed in tumor as compared to normal tissues, present in the pre-operation EVs and decreased in the post-operation EVs in each RNA biotype. The levels of 63 mRNAs, 3 lncRNAs, 2 miRNAs and 1 piRNA were significantly increased in the tumors and decreased in the post-operation urinary EVs, thus suggesting that these RNAs mainly originate from PC tissue. No such RNA biomarkers were identified in plasma EVs. This suggests that the fraction of PC-derived EVs in urine is larger than in plasma and allows the detection and tracking of PC-derived RNAs.en
dc.description.statusPeer reviewed
dc.format.extent4133348
dc.identifier.citationBajo-Santos, C, Brokāne, A, Zayakin, P, Endzeliņš, E, Soboļevska, K, Belovs, A, Jansons, J, Sperga, M, Llorente, A, Radoviča-Spalviņa, I, Lietuvietis, V & Linē, A 2023, 'Plasma and urinary extracellular vesicles as a source of RNA biomarkers for prostate cancer in liquid biopsies', Frontiers in Molecular Biosciences, vol. 10, 980433. https://doi.org/10.3389/fmolb.2023.980433
dc.identifier.doi10.3389/fmolb.2023.980433
dc.identifier.issn2296-889X
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/15150
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85148382421&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofFrontiers in Molecular Biosciences
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectdigital droplet PCR
dc.subjectextracellular vesicles
dc.subjectliquid biopsy
dc.subjectprostate cancer
dc.subjectRNA biotypes
dc.subjectRNA sequencing
dc.subject3.2 Clinical medicine
dc.subject1.6 Biological sciences
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectBiochemistry
dc.subjectMolecular Biology
dc.subjectBiochemistry, Genetics and Molecular Biology (miscellaneous)
dc.subjectSDG 3 - Good Health and Well-being
dc.titlePlasma and urinary extracellular vesicles as a source of RNA biomarkers for prostate cancer in liquid biopsiesen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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