Staphylococcus sugu biofilmas veidošanas nozīme ar peritoneālo dialīzi asociētu peritonītu gadījumā
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Date
2021
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Rīgas Stradiņa universitāte
Rīga Stradiņš University
Rīga Stradiņš University
Abstract
Staphylococcus sugu biofilmas veidošanas nozīme ar peritoneālo dialīzi asociētu peritonītu gadījumā
Ievads. Peritoneālā dialīze (PD) ir izplatīta nieru aizstājterapijas metode. Kā galveno PD komplikāciju var minēt infekciju slimības, kas ir kā būtisks cēlonis šīs nieru aizstājterapijas metodes pārtraukšanai vai ir kā viens no galvenajiem mirstības cēloņiem PD pacientiem. Staphylococcus sugas ir biežs iemesls ar PD asociēta peritonīta attīstībā. Šīm baktērijām ir plašs virulences profils un spēcīga biofilmu veidošanas spēja. Biofilma ir ievērojama barjera, kura pazemina antibakteriālo vielu ietekmi uz tajā iekļautajām baktērijām, kā arī novērš saimniekorganisma imūno atbildi uz šiem mikororganismiem.
Materiāli un metodes. Pētījuma mērķis bija noteikt Staphylococcus sugu biofilmu veidošanās spēju, kas tika izolētas no PD pacientiem ar peritonītu. Kopumā tika izdalītas 11 Staphylococcus kultūras no PD pacientiem, kam bija attīstījusies ar PD asociēta infekcija un kas ārstējās Paula Stradiņa Klīniskā universitātes slimnīcā. Baktēriju identifikācija tika veikta ar MALDI-TOF vai VITEK-2 sistēmu. Antibakteriālā jutība tika noteikta saskaņā ar EUCAST V11.0 standartu. Baktēriju spēju veidot biofilmu tika noteikta, izmantojot 96 bedrīšu mikroplati. Sekojoši baktēriju biofilmas optiskais blīvums (OD) tika mērīts ar mikroplates spektrofotometru (TECAN INFINITE F50) pie 570 nm viļņa garuma. Dati tika analizēti, izmantojot Microsoft Excel 10.
Rezultāti: Baktēriju celmi tika izolēti no 9 peritoneālās dialīzes pacientiem (4 dializāti, 7 uztriepes no peritoneālās dialīzes katetra izejas atveres). Būtiska biofilmas veidošanas spēja tika novērota 8 izolētajiem baktēriju celmiem. Visbiežāk Staphylococcus celmi uzrādīja vidēju biofilmas veidošanas spēju (6; 55%), spēcīgu (2; 18%) un neveidoja vai veidoja ļoti minimāli (3; 27%). Pieciem izolētiem celmiem bija rezistence pret penicilīnu G, viens celms bija rezistents pret ampicilīnu. Viens no S. aureus celmiem bija rezistents pret meticilīnu un uzrādīja spēcīgu biofilmu veidošanas spēju.
Secinājumi. Izdalītie Staphylococcu celmi uzrādīja biežu biofilmu veidošanas spēju un līdz ar to tai ir svarīga loma pie ar PD asociēta peritonīta attīstībā. Antibakteriālā rezistence un biofilmu veidošana ir nozīmīgi faktori, kas ietekmē Ar PD asociēta peritonīta ārstēšanu un vispārējo iznākumu.
Impact of Staphylococcus species biofilm formation in peritoneal dialysis associated peritonitis. Introduction. Peritoneal dialysis (PD) is a common method of renal replacement therapy. Infection as PD associated peritonitis is a major complication and cause of dialysis failure and is one of the leading causes of mortality. Frequently Staphylococcus species are the cause of PD associated peritonitis. These bacteria have wide profile of virulence and strong ability of biofilm formation, it is a significant barrier which lowers the penetration of antibacterial substances and prevents bacteria against host immune system. Materials and methods. The aim of the study was to determine biofilm formation ability of Staphylococcus species isolated from PD patients with the peritonitis. In total 11 Staphylococcus species were obtained from PD patients with PD associated infections at Pauls Stradins Clinical University Hospital. Bacterial identification was performed with MALDI-TOF or VITEK-2 system. Antimicrobial susceptibility was performed according to EUCAST V11.0 standard. Biofilm growth was detected using microtiter plate assay. Afterwards, the optical density (OD) of bacterial biomass was measured with a microplate spectrophotometer (TECAN INFINITE F50) at 570 nm wavelength. Data were analysed using Microsoft Excel 10. Results. Bacterial strains were isolated from 9 Peritoneal dialysis patients (4 dialysate,7 smears from peritoneal dialysis catheter exit site). Relevant biofilm production was observed among 8 isolated strains. Most commonly, strains produced moderate biofilm (6; 55%), strong (2; 18%) and non or weak (3; 27%). Five isolated strains showed resistance to penicillin G, one strain was resistant to ampicillin. One of S. aureus strains was methicillin resistant and produced strong biofilm. Conclusions. Biofilm formation was detected commonly in isolated bacterial strains and plays an important role in the development of peritonitis. Antibacterial resistance and biofilm formation is an important factor of peritonitis treatment and overall prognosis.
Impact of Staphylococcus species biofilm formation in peritoneal dialysis associated peritonitis. Introduction. Peritoneal dialysis (PD) is a common method of renal replacement therapy. Infection as PD associated peritonitis is a major complication and cause of dialysis failure and is one of the leading causes of mortality. Frequently Staphylococcus species are the cause of PD associated peritonitis. These bacteria have wide profile of virulence and strong ability of biofilm formation, it is a significant barrier which lowers the penetration of antibacterial substances and prevents bacteria against host immune system. Materials and methods. The aim of the study was to determine biofilm formation ability of Staphylococcus species isolated from PD patients with the peritonitis. In total 11 Staphylococcus species were obtained from PD patients with PD associated infections at Pauls Stradins Clinical University Hospital. Bacterial identification was performed with MALDI-TOF or VITEK-2 system. Antimicrobial susceptibility was performed according to EUCAST V11.0 standard. Biofilm growth was detected using microtiter plate assay. Afterwards, the optical density (OD) of bacterial biomass was measured with a microplate spectrophotometer (TECAN INFINITE F50) at 570 nm wavelength. Data were analysed using Microsoft Excel 10. Results. Bacterial strains were isolated from 9 Peritoneal dialysis patients (4 dialysate,7 smears from peritoneal dialysis catheter exit site). Relevant biofilm production was observed among 8 isolated strains. Most commonly, strains produced moderate biofilm (6; 55%), strong (2; 18%) and non or weak (3; 27%). Five isolated strains showed resistance to penicillin G, one strain was resistant to ampicillin. One of S. aureus strains was methicillin resistant and produced strong biofilm. Conclusions. Biofilm formation was detected commonly in isolated bacterial strains and plays an important role in the development of peritonitis. Antibacterial resistance and biofilm formation is an important factor of peritonitis treatment and overall prognosis.
Description
Medicīna
Medicine
Veselības aprūpe
Health Care
Medicine
Veselības aprūpe
Health Care
Keywords
peritoneālā dialīze; Staphylococcus infekcija; biofilmu veidošana, peritoneal dialysis; Staphylococcus infection; biofilm production