Development of a real-time PCR method for rapid diagnosis of canine babesiosis and anaplasmosis

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dc.contributor.authorKivrane, Agnija
dc.contributor.authorNamina, Agne
dc.contributor.authorSeleznova, Maija
dc.contributor.authorAkopjana, Sarmite
dc.contributor.authorCapligina, Valentina
dc.contributor.authorRanka, Renate
dc.date.accessioned2022-01-26T19:00:02Z
dc.date.available2022-01-26T19:00:02Z
dc.date.issued2021-12
dc.descriptionFunding Information: This work was supported by the ERDF project no. 1.1.1.1/16/A/044. The funders of the study had no role in the study design, analysis or writing of the report. Publisher Copyright: © 2021, The Author(s).
dc.description.abstractBackground: Canine babesiosis and anaplasmosis, caused by Babesia canis and Anaplasma phagocytophilum, respectively, are significant tick-borne diseases in Baltic countries. Both diseases can be diagnosed on the basis of clinicopathological findings, by direct pathogen detection in blood smears or by indirect pathogen detection; however, because of high selectivity and specificity, molecular methods may be advantageous. The goal of this study was to develop a duplex real-time polymerase chain reaction (RT-PCR) method for the detection of B. canis and A. phagocytophilum in canine clinical samples. Methods: Sequence-based polymorphism analysis of genes encoding B. canis-specific merozoite surface protein Bc28.1 (Bc28.1) and A. phagocytophilum malate dehydrogenase (mdh) was performed on pathogen isolates present in Latvian domestic dogs. The obtained results were used to design a species-specific duplex RT-PCR assay. Results: The presence of three B. canis Bc28.1 gene sequence types was revealed in canine samples with a nonuniform geographical distribution, and two types of A. phagocytophilum mdh genes were detected. The novel duplex RT-PCR assay provided correct classification of samples positive and negative for B. canis and A. phagocytophilum. The analytical sensitivity of this assay was ten gene copies/ reaction for both pathogens. Conclusions: A novel duplex RT-PCR molecular method was developed for the detection of B. canis and A. phagocytophilum in canine clinical samples. Sequence variability of Bc28.1 and mdh genes indicated the genetic variability of B. canis and A. phagocytophilum isolates occurring in Latvian domestic dogs. Graphic Abstract: [Figure not available: see fulltext.].en
dc.description.statusPeer reviewed
dc.format.extent1597708
dc.identifier.citationKivrane, A, Namina, A, Seleznova, M, Akopjana, S, Capligina, V & Ranka, R 2021, 'Development of a real-time PCR method for rapid diagnosis of canine babesiosis and anaplasmosis', Parasites and Vectors, vol. 14, no. 1, 266. https://doi.org/10.1186/s13071-021-04756-9
dc.identifier.doi10.1186/s13071-021-04756-9
dc.identifier.issn1756-3305
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/7307
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85106577059&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofParasites and Vectors
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAnaplasma phagocytophilum
dc.subjectBabesia canis
dc.subjectBc28.1 gene
dc.subjectGenotyping
dc.subjectMdh gene
dc.subjectReal-time PCR
dc.subject1.6 Biological sciences
dc.subject3.3 Health sciences
dc.subject4.3 Veterinary science
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectParasitology
dc.subjectInfectious Diseases
dc.subjectSDG 3 - Good Health and Well-being
dc.titleDevelopment of a real-time PCR method for rapid diagnosis of canine babesiosis and anaplasmosisen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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