Analysis of the Spatiotemporal Development of Hematopoietic Stem and Progenitor Cells in the Early Human Embryo

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dc.contributor.authorEasterbrook, Jennifer
dc.contributor.authorRybtsov, Stanislav
dc.contributor.authorGordon-Keylock, Sabrina
dc.contributor.authorIvanovs, Andrejs
dc.contributor.authorTaoudi, Samir
dc.contributor.authorAnderson, Richard A.
dc.contributor.authorMedvinsky, Alexander
dc.date.accessioned2021-04-16T06:35:01Z
dc.date.available2021-04-16T06:35:01Z
dc.date.issued2019-05-14
dc.descriptionFunding Information: We thank the patients for donating tissue and A. Saunderson, J. Creiger, and N. Forson for gaining consent from patients and collecting specimens. We thank J. Verth, C. Manson, and their staff for animal maintenance. This work was supported by the Wellcome Trust (grant 100468/Z/12/Z , J.E.) and the MRC (grant G0901577 , A.M.). Publisher Copyright: © 2019
dc.description.abstractDefinitive hematopoietic stem cells (HSCs)first emerge in the aorta-gonad-mesonephros (AGM)region in both mice and humans. An ex vivo culture approach has enabled recapitulation and analysis of murine HSC development. Knowledge of early human HSC development is hampered by scarcity of tissue: analysis of both CFU-C and HSC development in the human embryo is limited. Here, we characterized the spatial distribution and temporal kinetics of CFU-C development within early human embryonic tissues. We then sought to adapt the murine ex vivo culture system to recapitulate human HSC development. We show robust expansion of CFU-Cs and maintenance, but no significant expansion, of human HSCs in culture. Furthermore, we demonstrate that HSCs emerge predominantly in the middle section of the dorsal aorta in our culture system. We conclude that there are important differences between early mouse and human hematopoiesis, which currently hinder the quest to recapitulate human HSC development ex vivo.en
dc.description.statusPeer reviewed
dc.format.extent13
dc.format.extent1944067
dc.identifier.citationEasterbrook, J, Rybtsov, S, Gordon-Keylock, S, Ivanovs, A, Taoudi, S, Anderson, R A & Medvinsky, A 2019, 'Analysis of the Spatiotemporal Development of Hematopoietic Stem and Progenitor Cells in the Early Human Embryo', Stem Cell Reports, vol. 12, no. 5, pp. 1056-1068. https://doi.org/10.1016/j.stemcr.2019.03.003
dc.identifier.doi10.1016/j.stemcr.2019.03.003
dc.identifier.issn2213-6711
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/3817
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85065158624&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofStem Cell Reports
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectfetal liver
dc.subjecthematopoietic progenitors
dc.subjecthematopoietic stem cells
dc.subjecthuman embryo
dc.subjectyolk sac
dc.subject3.1 Basic medicine
dc.subject3.2 Clinical medicine
dc.subject1.6 Biological sciences
dc.subject3.3 Health sciences
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectBiochemistry
dc.subjectGenetics
dc.subjectDevelopmental Biology
dc.subjectCell Biology
dc.subjectSDG 3 - Good Health and Well-being
dc.titleAnalysis of the Spatiotemporal Development of Hematopoietic Stem and Progenitor Cells in the Early Human Embryoen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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