Toxicity of mercury to hybridoma TA7 cells

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dc.contributor.authorRemez, Inessa
dc.contributor.authorAndersons, Pauls
dc.contributor.authorVeksler, Hackel
dc.date.accessioned2021-09-20T08:10:01Z
dc.date.available2021-09-20T08:10:01Z
dc.date.issued1999
dc.description.abstractEnvironmental mercury and mercury compound contamination has increased dramatically since the industrial revolution. This paper describes the toxic effects of mercury on a culture of hybridoma TA7 cells, which produce antibodies against the A-subunit of viskumin. Cells were cultivated on 96- well flat-bottomed plates with RPMI-1640 medium supplemented with 10% fetal calf serum at 37°C in 5% CO2/95% air. The cells were exposed to 0.1nM/l- 10μM/l Hg2(NO3)2·2H2O (mercury nitrate) during the exponential growth phase. Toxicity was assessed by using the colorimetric MTT (tetrazolium) assay after exposure for 48 hours. Cell growth and cell survival were evaluated by using percentage indices of cellular content in exposed cells when compared to non-exposed control cells. The concentrations of the no- effect level, the lowest observed effect level and the the highest toxic effect level were registered. The toxic effects of the mercury compound on the hybridoma cells occurred between 0.1μM/l and 10μM/l.en
dc.description.statusPeer reviewed
dc.format.extent5
dc.format.extent63784
dc.identifier.citationRemez, I, Andersons, P & Veksler, H 1999, 'Toxicity of mercury to hybridoma TA7 cells', ATLA Alternatives to Laboratory Animals, vol. 27, no. 3, pp. 397-401. https://doi.org/10.1177/026119299902700315
dc.identifier.doi10.1177/026119299902700315
dc.identifier.issn0261-1929
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/6388
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=0033055794&partnerID=8YFLogxK
dc.identifier.urlhttps://journals-sagepub-com.db.rsu.lv/doi/abs/10.1177/026119299902700315
dc.language.isoeng
dc.relation.ispartofATLA Alternatives to Laboratory Animals
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectDose-effect
dc.subjectHybridoma TA7 cells
dc.subjectMercury
dc.subjectToxicity
dc.subjectGeneral Biochemistry,Genetics and Molecular Biology
dc.subjectToxicology
dc.subjectMedical Laboratory Technology
dc.titleToxicity of mercury to hybridoma TA7 cellsen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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