Identification of Inflammatory and Regulatory Cytokines IL-1α-, IL-4-, IL-6-, IL-12-, IL-13-, IL-17A-, TNF-α-, and IFN-γ-Producing Cells in the Milk of Dairy Cows with Subclinical and Clinical Mastitis

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dc.contributor.authorVitenberga-Verza, Zane
dc.contributor.authorPilmane, Māra
dc.contributor.authorŠerstņova, Ksenija
dc.contributor.authorMelderis, Ivars
dc.contributor.authorGontar, Łukasz
dc.contributor.authorKochanski, Maksymilian
dc.contributor.authorDrutowska, Andzelika
dc.contributor.authorGergely, Maroti
dc.contributor.authorPrieto-Simona, Beatrise
dc.contributor.institutionInstitute of Anatomy and Anthropology
dc.date.accessioned2022-08-16T21:02:43Z
dc.date.available2022-08-16T21:02:43Z
dc.date.issued2022-03-17
dc.descriptionFunding Information: The project is implemented under ERA-NET ICRAD (International Coordination of Research on Infectious Diseases) and co-financing of Spanish State Research Agency, Hungarian National Food Chain Safety Office, Polish National Centre for Research and Development, and Latvian State Education Development Agency. This project has received funding from the European Union?s Horizon 2020 research and innovation program under grant agreement no. 862605. Funding Information: Funding: The project is implemented under ERA-NET ICRAD (International Coordination of Research on Infectious Diseases) and co-financing of Spanish State Research Agency, Hungarian National Food Chain Safety Office, Polish National Centre for Research and Development, and Latvian State Education Development Agency. This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement no. 862605. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
dc.description.abstractIn naturally occurring bovine mastitis, effects of infection depend on the host inflammatory response, including the effects of secreted cytokines. Knowledge about the inflammatory and regulatory cytokines in milk cells of free-stall barn dairy cows and in naturally occurring mastitis is lacking as most studies focus on induced mastitis. Hereby, the aim of the study was to determine inflammatory and regulatory cytokines in the milk of dairy cows with subclinical and clinical mastitis. The following examinations of milk samples were performed: differential counting of somatic cells (SCC), bacteriological examination, and immunocytochemical analysis. Mean SCC increased in subclinical and clinical mastitis cases. The number of pathogenic mastitis-causing bacteria on plates increased in subclinical mastitis cases but decreased in clinical mastitis. The inflammatory and regulatory markers in the milk cells of healthy cows showed the highest mean cell numbers (%). In mastitis cases, immunoreactivity was more pronounced for IL-4, IL-6, IL-12, IL-13, IL-17A, TNF-, and IFN-y. Data about subclinical and clinical mastitis demonstrate inflammatory responses to intramammary infection driven by IL-1, IL-4, and IL-17A. Moreover, the host defense response in mastitis is characterized by continuation or resolution of initial inflammation. IL-12 and INF-immunoreactivity was recognized to differ mastitis cases from the relative health status.en
dc.description.statusPeer reviewed
dc.format.extent23
dc.format.extent2878644
dc.identifier.citationVitenberga-Verza, Z, Pilmane, M, Šerstņova, K, Melderis, I, Gontar, Ł, Kochanski, M, Drutowska, A, Gergely, M & Prieto-Simona, B 2022, 'Identification of Inflammatory and Regulatory Cytokines IL-1α-, IL-4-, IL-6-, IL-12-, IL-13-, IL-17A-, TNF-α-, and IFN-γ-Producing Cells in the Milk of Dairy Cows with Subclinical and Clinical Mastitis', Pathogens, vol. 11, no. 3, 372. https://doi.org/10.3390/pathogens11030372
dc.identifier.doi10.3390/pathogens11030372
dc.identifier.issn2076-0817
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/9390
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85126985715&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofPathogens
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectdiagnosis
dc.subjectmastitis
dc.subjectbovine
dc.subjectcytokines
dc.subjectinflammation
dc.subjectimmunohistochemistry
dc.subjectmilk
dc.subject3.1 Basic medicine
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.titleIdentification of Inflammatory and Regulatory Cytokines IL-1α-, IL-4-, IL-6-, IL-12-, IL-13-, IL-17A-, TNF-α-, and IFN-γ-Producing Cells in the Milk of Dairy Cows with Subclinical and Clinical Mastitisen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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