Identifying the Microbiome of the Adenoid Surface of Children Suffering from Otitis Media with Effusion and Children without Middle Ear Effusion Using 16S rRNA Genetic Sequencing

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dc.contributor.authorSokolovs-Karijs, Oļegs
dc.contributor.authorBriviba, Monta
dc.contributor.authorSaksis, Rihards
dc.contributor.authorRozenberga, Maija
dc.contributor.authorGirotto , Francesca
dc.contributor.authorOsīte, Jana
dc.contributor.authorReinis, Aigars
dc.contributor.authorSumeraga, Gunta
dc.contributor.authorKrūmiņa, Angelika
dc.contributor.institutionDepartment of Otorhinolaryngology
dc.contributor.institutionDepartment of Infectology
dc.contributor.institutionDepartment of Biology and Microbiology
dc.date.accessioned2023-08-23T08:30:01Z
dc.date.available2023-08-23T08:30:01Z
dc.date.issued2023-08
dc.descriptionFunding Information: Research funded by “Centrālā Laboratorija”, further information upon request. During the manuscript preparation and review process, Rihards Saksis was supported by the project “Strengthening of the capacity of doctoral studies at the University of Latvia within the framework of the new doctoral model”, identification No.8.2.2.0/20/I/006. Publisher Copyright: © 2023 by the authors.
dc.description.abstractBackground: The upper respiratory tract harbors diverse communities of commensal, symbiotic, and pathogenic organisms, originating from both the oral and nasopharyngeal microbiota. Among the primary sites of microbial colonization in the upper airways are the adenoids. Alterations in the adenoid microbiota have been implicated in the development of various conditions, including secretory otitis media. Aim: This study aims to employ 16S rRNA genetic sequencing to identify the most common bacteria present on the surface of adenoids in children with otitis media with effusion and compare them with children without pathologies in the tympanic cavity. Additionally, we seek to determine and compare the bacterial diversity in these two study groups. Materials and Methods: A total of nineteen samples from the adenoid surfaces were collected, comprising two groups: thirteen samples from children without middle ear effusion and six samples from children with secretory otitis media. The libraries of the V3–V4 hypervariable region of the bacterial 16S rRNA gene was made and sequenced using MiSeq platform. Results: The most prevalent phyla observed in both groups were Proteobacteria, Firmicutes, and Bacteroidetes. The most common bacterial genera identified in both groups were Haemophilus, Streptococcus, Moraxella, Fusobacterium, and Bordetella, with Fusobacterium and Moraxella being more prevalent in the groups that had no middle ear effusion, while Haemophulus and Streptococcus were more prevalent in the otitis media with effusion group, although not in a statistically significant way. Statistical analysis shows a trend towards bacterial composition and beta diversity being similar between the study groups; however, due to the limited sample size and unevenness between groups, we should approach this data with caution. Conclusion: The lack of prolific difference in bacterial composition between the study groups suggests that the role of the adenoid microbiome in the development of otitis media with effusion may be less significant.en
dc.description.statusPeer reviewed
dc.format.extent13
dc.format.extent2108188
dc.identifier.citationSokolovs-Karijs, O, Briviba, M, Saksis, R, Rozenberga, M, Girotto , F, Osīte, J, Reinis, A, Sumeraga, G & Krūmiņa, A 2023, 'Identifying the Microbiome of the Adenoid Surface of Children Suffering from Otitis Media with Effusion and Children without Middle Ear Effusion Using 16S rRNA Genetic Sequencing', Microorganisms, vol. 11, no. 8, 1955. https://doi.org/10.3390/microorganisms11081955
dc.identifier.doi10.3390/microorganisms11081955
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/14844
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85168897345&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofMicroorganisms
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBacteria
dc.subjectadenoids
dc.subject16s rRNA gene sequencing
dc.subjectmicrobiome
dc.subjectotitis media
dc.subject3.2 Clinical medicine
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.titleIdentifying the Microbiome of the Adenoid Surface of Children Suffering from Otitis Media with Effusion and Children without Middle Ear Effusion Using 16S rRNA Genetic Sequencingen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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