Serology in the Digital Age : Using Long Synthetic Peptides Created from Nucleic Acid Sequences as Antigens in Microarrays

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dc.contributor.authorRizwan, Muhammad
dc.contributor.authorRönnberg, Bengt
dc.contributor.authorCistjakovs, Maksims
dc.contributor.authorLundkvist, Åke
dc.contributor.authorPipkorn, Rudiger
dc.contributor.authorBlomberg, Jonas
dc.contributor.institutionInstitute of Microbiology and Virology
dc.date.accessioned2021-07-02T15:25:01Z
dc.date.available2021-07-02T15:25:01Z
dc.date.issued2016-08-10
dc.description.abstractBACKGROUND: Antibodies to microbes, or to autoantigens, are important markers of disease. Antibody detection (serology) can reveal both past and recent infections. There is a great need for development of rational ways of detecting and quantifying antibodies, both for humans and animals. Traditionally, serology using synthetic antigens covers linear epitopes using up to 30 amino acid peptides. METHODS: We here report that peptides of 100 amino acids or longer ("megapeptides"), designed and synthesized for optimal serological performance, can successfully be used as detection antigens in a suspension multiplex immunoassay (SMIA). Megapeptides can quickly be created just from pathogen sequences. A combination of rational sequencing and bioinformatic routines for definition of diagnostically-relevant antigens can, thus, rapidly yield efficient serological diagnostic tools for an emerging infectious pathogen. RESULTS: We designed megapeptides using bioinformatics and viral genome sequences. These long peptides were tested as antigens for the presence of antibodies in human serum to the filo-, herpes-, and polyoma virus families in a multiplex microarray system. All of these virus families contain recently discovered or emerging infectious viruses. CONCLUSION: Long synthetic peptides can be useful as serological diagnostic antigens, serving as biomarkers, in suspension microarrays.en
dc.description.statusPeer reviewed
dc.format.extent1971410
dc.identifier.citationRizwan, M, Rönnberg, B, Cistjakovs, M, Lundkvist, Å, Pipkorn, R & Blomberg, J 2016, 'Serology in the Digital Age : Using Long Synthetic Peptides Created from Nucleic Acid Sequences as Antigens in Microarrays', Microarrays (Basel, Switzerland), vol. 5, no. 3. https://doi.org/10.3390/microarrays5030022
dc.identifier.doi10.3390/microarrays5030022
dc.identifier.issn2076-3905
dc.identifier.otherPubMedCentral: PMC5040969
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/5824
dc.language.isoeng
dc.relation.ispartofMicroarrays (Basel, Switzerland)
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subject3.1 Basic medicine
dc.subject3.2 Clinical medicine
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectSDG 3 - Good Health and Well-being
dc.titleSerology in the Digital Age : Using Long Synthetic Peptides Created from Nucleic Acid Sequences as Antigens in Microarraysen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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