Heart-Type Fatty Acid Binding Protein Binds Long-Chain Acylcarnitines and Protects against Lipotoxicity

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dc.contributor.authorZelencova-Gopejenko, Diana
dc.contributor.authorVideja, Melita
dc.contributor.authorGrandane, Aiga
dc.contributor.authorPudnika-Okinčica, Linda
dc.contributor.authorSipola, Anda
dc.contributor.authorVilks, Karlis
dc.contributor.authorDambrova, Maija
dc.contributor.authorJaudzems, Kristaps
dc.contributor.authorLiepinsh, Edgars
dc.contributor.institutionFaculty of Pharmacy
dc.date.accessioned2023-04-05T08:20:01Z
dc.date.available2023-04-05T08:20:01Z
dc.date.issued2023-03-14
dc.descriptionFunding Information: This research was funded by the European Union’s Horizon 2020 research and innovation program project FAT4BRAIN under grant agreement No. 857394 and by Latvian Institute of Organic Synthesis internal student grants IG-2022-04 and IG-2023-04 (to D.Z.-G.). Publisher Copyright: © 2023 by the authors.
dc.description.abstractHeart-type fatty-acid binding protein (FABP3) is an essential cytosolic lipid transport protein found in cardiomyocytes. FABP3 binds fatty acids (FAs) reversibly and with high affinity. Acylcarnitines (ACs) are an esterified form of FAs that play an important role in cellular energy metabolism. However, an increased concentration of ACs can exert detrimental effects on cardiac mitochondria and lead to severe cardiac damage. In the present study, we evaluated the ability of FABP3 to bind long-chain ACs (LCACs) and protect cells from their harmful effects. We characterized the novel binding mechanism between FABP3 and LCACs by a cytotoxicity assay, nuclear magnetic resonance, and isothermal titration calorimetry. Our data demonstrate that FABP3 is capable of binding both FAs and LCACs as well as decreasing the cytotoxicity of LCACs. Our findings reveal that LCACs and FAs compete for the binding site of FABP3. Thus, the protective mechanism of FABP3 is found to be concentration dependent.en
dc.description.statusPeer reviewed
dc.format.extent4314455
dc.identifier.citationZelencova-Gopejenko, D, Videja, M, Grandane, A, Pudnika-Okinčica, L, Sipola, A, Vilks, K, Dambrova, M, Jaudzems, K & Liepinsh, E 2023, 'Heart-Type Fatty Acid Binding Protein Binds Long-Chain Acylcarnitines and Protects against Lipotoxicity', International Journal of Molecular Sciences, vol. 24, no. 6, 5528. https://doi.org/10.3390/ijms24065528
dc.identifier.doi10.3390/ijms24065528
dc.identifier.issn1661-6596
dc.identifier.otherPubMedCentral: PMC10058761
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/11283
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85151108933&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectFatty Acid Binding Protein 3/metabolism
dc.subjectFatty Acid-Binding Proteins/metabolism
dc.subjectFatty Acids pharmacology
dc.subjectCarnitine
dc.subjectMyocytes
dc.subjectCardiac metabolism
dc.subjectisothermal titration calorimetry
dc.subjectlong-chain acylcarnitines
dc.subject1.6 Biological sciences
dc.subject3.1 Basic medicine
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.titleHeart-Type Fatty Acid Binding Protein Binds Long-Chain Acylcarnitines and Protects against Lipotoxicityen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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