EC funded resources / Publikācijas EK projektu ietvaros

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Browsing EC funded resources / Publikācijas EK projektu ietvaros by Subject "autoimmunity"

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    International Conference “Autoimmune Diseases: Main Problems and Solutions” (November 9–10, 2023 Riga). Abstract Book
    (Rīga Stradiņš University, 2023-11) University of Ferrara, Italy; Ulm University, Germany; Zabludowicz Center for Autoimmune Diseases at Sheba Medical Center, Israel
    Conference is organized by the Rīga Stradiņš University within the frame of the EC Horizon 2020 Framework program project “Reducing networking gaps between Rīga Stradiņš University (RSU) and internationally-leading counterparts in viral infection-induced autoimmunity research (VirA)”. The aim of the Conference is to bring together researchers exploring triggers and mechanisms of autoimmunity, leading to better understanding of chronic diseases and comorbidities in order to deliver precise and early diagnostic and to move towards the development of personalized medicine. The scientific program of the Conference covers different aspects of autoimmunity in plenary lectures, oral presentations and poster sessions. The Conference has attracted about 100 participants from 7 countries.
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    Monocytes/Macrophages Act as Mediators for Human Herpesvirus-6 Infection of Thyroid Gland in Patients with Autoimmune Thyroiditis
    (2019) Sokolovska, Lība; Sultanova, Alina; Čistjakovs, Maksims; Cunskis, Egils; Murovska, Modra
    The aim of this study was to investigate the possibility of using monocytes/macrophages as mediators in human herpesvirus-6 (HHV-6) infection of thyroid gland tissues in autoimmune thyroiditis (AIT). Seventy-three AIT patients were enrolled in this study. The control group consisted of 80 blood donors. Monocyte/macrophage isolation for AIT patient samples was performed by adherence. HHV-6 was detected in peripheral blood mononuclear cell (PBMC) DNA samples using nested polymerase chain reaction (nPCR). Gene expression of HHV-6 active infection marker (U79/80) and chemokine receptors (U12, U51) in patient monocyte/macrophage samples and blood donor PBMC samples was detected using reverse-transcription PCR. HHV-6 viral load was detected by using quantitative-PCR technique. The HHV-6 genomic sequence was found significantly more frequently among AIT patient than control group samples. Markers of active infection were found in 8 AIT patient monocyte/macrophage samples (11%) and in none of control group PBMC samples. HHV-6 U51 mRNA expression was detected only in AIT patient samples (2/24 previously positive for HHV-6). Since HHV-6 genomic sequences were found significantly more frequently in AIT patient samples and active infection markers were found in patient monocytes/macrophages, our results suggest that monocytes/macrophages may be used by HHV-6 as mediators for thyroid gland infection.
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    Possible Involvement of Human Herpesvirus-6 U83 Gene Expression in Autoimmune Thyroiditis Development
    (2019) Sultanova, Alina; Čistjakovs, Maksims; Sokolovska, Lība; Cunskis, Egils; Murovska, Modra
    Viral infections have been frequently cited as important environmental factors implicated in autoimmune thyroiditis (AIT) development, although no specific virus has yet been conclusively associated with the disease. Some evidence implicates human herpesvirus-6 (HHV-6) in this disease. The aim of this study was to investigate the role of the HHV-6 U83 gene expression in autoimmune thyroiditis development. Fifty-one patients with AIT following thyroidectomy and a control group of 30 autopsied subjects without thyroid pathologies for comparing virology results and 30 healthy blood donors for comparing serology results were enrolled in this study. HHV-6 U83 gene expression was determined using nested PCR with complementary DNA as the template acquired from thyroid gland extracted RNA. Plasma samples of AIT patients and blood donors were tested for IL-2, IL-4, IL-10, sTNF-RII and IL-1beta levels by ELISA. Virology results were compared with pro- and anti-inflammatory cytokine levels to determine possible interaction of HHV-6 with host immune response. HHV-6 U83 gene expression was found only in 24% (12/49) of AIT patient thyroid gland tissue samples and in none of the control group individuals, showing possible involvement of this gene in AIT development. However, no interaction between HHV-6 and changes in cytokine levels was found.